eng
Mansoura University, Faculty of Agriculture
Journal of Plant Protection and Pathology
2090-3677
2090-3758
2019-02-01
10
2
97
100
10.21608/jppp.2019.40883
40883
Original Article
A New Record for Mangrove Plume Moth Cenoloba obliteralis (Walker, 1864) (Fam. Tineodidae : Lepidoptera ) Attacking Mangrove Plant in Saudi Arabia
U. Abu El-Ghiet
1
Plant Protection Dept., Desert Res. Center, Mataria, Cairo, Egypt.
The first record for Mangrove plume moth Cenoloba obliteralis (Walker) on infested fruit of mangrove Jazan Red Sea coasts, Saudi in March 2016. The larvae of mangrove plume moth C. obliteralis feeding on flowering buds and consumed the cotyledons of fruits. During this study, six sites of mangrove plant were screened for infestation by C. obliteralis through four seasons. We observed that, the flower buds were available at different seasons meanwhile fruits were available in spring and autumn. Female of C. obliteralis was laid eggs at the base of the flower buds as a separate egg, after egg hatching larvae feed on flowers before its open and navigate between the cluster flowers. The infected flowers were diagnostic by presence of larvae feces and holes which made by the larvae. The direct examination of fruits, showing that female was laid eggs on the location of fruits communication per plant. After egg hatching new emergence larvae was enter into fruits and it feeds on the content of cotyledons. The mature larva was exit from the weakest site in the cortex of the fruit (attaching site of both cotyledons). No more than a larva watched inside the fruit as well as no cocoon seen inside the fruit.
https://jppp.journals.ekb.eg/article_40883_cff9419fdb92fd1149459b9dea22ae5b.pdf
Mangrove
Cenoloba obliteralis
Jazan
new record
eng
Mansoura University, Faculty of Agriculture
Journal of Plant Protection and Pathology
2090-3677
2090-3758
2019-02-01
10
2
101
109
10.21608/jppp.2019.40886
40886
Original Article
Bacterial Contamination of Mobile Phones Healthcare Versus Non-Healthcare Workers at Mansoura City, Egypt
A. Mohamedin
amohamedin@hotmail.com
1
A. Elsayed
2
Halima Nashnoush
3
Botany Department, Faculty of Science, Mansoura University, Egypt
Botany Department, Faculty of Science, Mansoura University, Egypt
Botany Department, Faculty of Science, Mansoura University, Egypt
The objective of this study was to identify the bacteria harbored by mobile phones of healthcare workers and non-healthcare workers from Mansoura City, Dakahlia Governorate and to determine their antibiotic resistance patterns. A total of 300 mobile phone samples, 150 samples from different Mansoura City Hospitals as healthcare workers (HCWs) and 150 samples from Mansoura University as non-healthcare workers (non-HCWs) used for isolation of bacteria on enriched, differential and selective media. Results of HCWs samples tested were 31(20.6%) samples no growth and 119(79.3%) showed bacterial contamination. Gram-positive isolates were 62(52.1%) samples Staphylococcus species, 58(48.7%) Staph.aureus, 71(59.6%) Bacillus species and 8(6.7%) were Micrococcus species. Also, 37(31.0%) mobile phones had only one genus and 82(68.9%) with two or more different genera. On the other hand 13(10.9%) isolates of Gram-negative bacilli were recorded. The results of non-HCWs samples indicated that 8(5.3%) samples showed no growth and 142(94.6%) samples were contaminated with bacteria. Gram-positive isolates were 86(60.6%) samples Staphylococcus species, 85(59.9%) Staph.aureus, 87(61.3%) Bacillus species and 24(16.9%) were Micrococcus species. Also, 20(14.1%) mobile phones had only one genus and 122(85.9%) with two or more different genera. On the other hand, 29(20.4%) isolates of Gram-negative bacilli were obtained and confirmed the results by BD PHOENIX Device. The Gram-negative bacterial isolates were resistant to Amikacin and Ampicillin, and sensitive to Ciprofloxacin, Trimethoprim sulfamethoxazole and Gentamicin. Also, some of isolates were resistant to Kanamycin, Nalidixic acid; Chloramphenicol and Tetracycline except Pseudomonas aeruginosa and Acinetobacter baumannii which are resistant to all antibiotics except Pseudomonas aeruginosa which was sensitive to Ciprofloxacin.Staphylococcus aureus of HCWs and non-HCWs 143(54.78%) were examined for nine antibiotics, the results were 99.30% were resistant to Oxacillin and Methicillin also, 89.5% were resistant to Ampicillin while 96.5% were sensitive to Ciprofloxacin, Kanamycin, and 98.6% to Trimethoprim sulfamethoxazole, Cefoxthin and Vancomycin. Also, 48(33.5%) Staph.aureus were resistant to penicillin-G. To reduce or prevent the contamination of the hands and mobile phones, healthcare workers should apply the standard hygienic precautions after using phones.
https://jppp.journals.ekb.eg/article_40886_d8fffe6fd09e39d59a5e3fdce0630618.pdf
mobile phones
Bacterial contamination
Susceptibility to antibiotics
Mansoura city
eng
Mansoura University, Faculty of Agriculture
Journal of Plant Protection and Pathology
2090-3677
2090-3758
2019-02-01
10
2
111
113
10.21608/jppp.2019.40891
40891
Original Article
Effect of some Materials for Controlling Green Peach Aphid, Myzus persicae (Sulzer)
Afaf El-Wahab
1
Horia Abd El-Wahab
2
Neama Abdel- Hameed
3
Eftkhar Abohatab
4
Manal Hager
5
faculty of science, Al- Azhar University ( for Girls), ARE
Plant Protection Research Institute- Agriculture Research Center, ARE
Faculty of science, Al- Azhar University ( for Girls), ARE
Plant Protection Research Institute- Agriculture Research Center, ARE
Faculty of science, Al- Azhar University ( for Girls), ARE
Myzus persicae (Sulzer)is one of major insect pests which infest many vegetable crops especially potato plants. Some natural materials such as, neem oil, menthol oil and nano chitosan were used in controlling M. persicae. The results showed that, neem oil was the most effective compound in controlling M. persicae with LC50 63.60 ppm, followed by menthol oil then nano chitosan with LC50 100.26 and 101.62 ppm, respectively.
https://jppp.journals.ekb.eg/article_40891_581b2e0b1a9a4643b4796e220f73a9b1.pdf
eng
Mansoura University, Faculty of Agriculture
Journal of Plant Protection and Pathology
2090-3677
2090-3758
2019-02-01
10
2
115
119
10.21608/jppp.2019.40896
40896
Original Article
Isolation, Characterization and Insecticidal Activity of Methylene Chloride Extract of Cladosporium cladosporioides Secondary Metabolites against Aphis gossypii (Glov.)
N. Shaker
1
G. Ahmed
2
M. El-Sawy
3
H. Ibrahim
4
H. Ismail
5
Applied Organic Chemistry, Faculty of Science (Girls), Al-Azhar University
Plant Protection Research Institute, ARC, Dokki, Giza, Egypt.
Applied Organic Chemistry, Faculty of Science (Girls), Al-Azhar University
Plant Protection Research Institute, ARC, Dokki, Giza, Egypt.
Plant Protection Research Institute, ARC, Dokki, Giza, Egypt.
The cotton aphid, Aphis gossypii Glover is a serious plant pests causing serious damage to the cultivated crops. Entomopathogenic fungi were early used for controlling aphids. Cladosporium cladosporioides is one of the promising candidates fungus can be used as a microorganism or can be used as a source of toxins against insect pests. Present research studied the chemical constituents of methylene chloride extracts of C. cladosporioides. It revealed 17 volatile compounds when analyzed by GC/MS technique. Also, two major compounds were isolated and identified as Pentadecanoic acid methyl ester (Compound 1) and Lupeol (Compound 2). Furthermore, the insecticidal activity of methylene chloride extracts of C. cladosporioides was tested against both adults and nymphs of cotton aphid, A. gossypii. It showed the most effective against nymphs of LC50 of 212.2319 ppm, LC90 of 1407.5663 ppm and toxicity index of 100%, while, it showed LC50 of 276.75 ppm, LC90 of 1902.15 ppm and toxicity index of 76.69% against adult stage.
https://jppp.journals.ekb.eg/article_40896_b8fb45b96b90b5b406171484181febe1.pdf
Cladosporium cladosporioide
secondary metabolites
methylene chloride extracts
insecticidal activity and Aphis gossypii
eng
Mansoura University, Faculty of Agriculture
Journal of Plant Protection and Pathology
2090-3677
2090-3758
2019-02-01
10
2
121
126
10.21608/jppp.2019.40898
40898
Original Article
Efficacy of some Entomopathogenic Fungal Extracts and their Chemical Constituents as Alternative Bio-Pesticides against Onion Thrips, Thrips tabaci L.
Amany Saad
dramany83@gmail.com
1
M. Sergany
sergany@mans.mans.edu.eg
2
M. Mostafa
3
Dina Fathy
4
Plant Pathology Department, Faculty of Agriculture, Mansoura University, Mansoura 35516, Egypt.
Pesticides Department, Faculty of Agriculture, Mansoura University, Mansoura 35516, Egypt.
Plant Protection Research Institute, Agriculture Research Center (ARC), Dokki, Giza, 12618, Egypt.
Economic Entomology Department, Faculty of Agriculture, Mansoura University, Mansoura 35516, Egypt.
Three entomopathogenic fungal secondary metabolites of Metarhizium anisopliae, Paceliomyces fumosoroseus and Trichoderma longibrachiatum were extracted using two different organic solvent and thoroughly investigated as bio-insecticide against nymphs and adults of onion thrips, Thrips tabaci using spraying method technique. The toxicity index of LC50 values revealed that T. longibrachiatuom secondary metabolites extracts (chloroform and ethyl acetate) were the most effective against the two developmental stages (nymph stage and adult) of T. tabaci followed by M. anisopliae extracts and the least toxic one P. fuosoroseus extracts after 7-days of treatment. The chemical composition of the most promising extracts in each entomopathogenic fungi were analyzed using GC-MS technique. Thirty-five compounds belonging to six different classes of natural products were characterized.
https://jppp.journals.ekb.eg/article_40898_191a98e1172714b8c0b9b6880ad5536a.pdf
Entomopathogenic fungi
secondary metabolites
lufenuron
Bio-pesticides
Onion
Thrips tabaci
eng
Mansoura University, Faculty of Agriculture
Journal of Plant Protection and Pathology
2090-3677
2090-3758
2019-02-01
10
2
127
131
10.21608/jppp.2019.40903
40903
Original Article
Big Aggregations of the Ladybird Beetles at the Mediterranean Sea Shore, Baltim, Kafr El-Sheikh, Egypt
Amany Khalifa
1
A. Mesbah
2
Biological Control Department, Plant Protection Research Institute, Agricultural Research Center, Egypt
Biological Control Department, Plant Protection Research Institute, Agricultural Research Center, Egypt
The phenomenon of ladybird beetle (Coleoptera: Coccinellidae) aggregation was noticed during April, May and June, 2013 at the Mediterranean sea shore of Baltim city, Kafr El-Sheikh Governorate, Northern Delta, Egypt. The occurring coccinellid species were surveyed. The survey included the sandy sea shore and the adjacent vegetations; wheat, clover and weeds. Massive aggregations of the coccinellids were found along the sea coast for about 110 km, extending from Baltim city (Kafr El-Sheikh Governorate) up to Rasheed city (Beheira Governorate). Ten coccinellid species were recorded; Coccinella undecimpunctata L., C. novemnotata Herbst., C. septempunctata L.,Cydonia vicina isis Mul.,Hippodamia convergens Guerin- Meneville, H. tredecimpunctata L., H. variegata (Goeze), Hippodamia sp., Rodalia cardinalis Mul., and Scymnus spp. The phenomenon didn’t occur in year 2014 but re-occurred in year 2015 and again disappeared in 2016. The most dominant species was C. undecimpunctata (90.55 and 86.84%), followed by C. novemnotata (6.30 and 12.39%) in 2013 and 2015, respectively. All other coccinellid species were collected in little numbers. In 2013, the majority of coccinellids (46.30and 18.74%) were recorded by late April and3rd week of May, respectively. In the same year, the wheat plantations harbored 75.15%. C. septempunctata, followed by C. undecimpunctata (17.52%) and H. tredecimpunctata (6.53%). In clover, the most dominant species was C. undecimpunctata (51.71%), followed by H. tredecimpunctata (17.11%), and then Hippodamia spp. (12.63%). In weeds, C. undecimpunctata was the most occurring (42.73%), followed by Scymnus spp. (29%). In 2015, C. undecimpunctata was the most dominant in wheat, clover and weeds, with values of 90.49, 80.71 and 49.92%, respectively. C. vicina isis and Scymnus spp.were detected only in weeds
https://jppp.journals.ekb.eg/article_40903_93fb8c91a484b727feaa82ef362695df.pdf
ladybird beetles
Aggregation
Mediterranean Sea Shore
Egypt
eng
Mansoura University, Faculty of Agriculture
Journal of Plant Protection and Pathology
2090-3677
2090-3758
2019-02-01
10
2
133
136
10.21608/jppp.2019.40907
40907
Original Article
Effect of Insect Infestation on some Internal Components of some Important Cactus Plants
Samia Yassin
1
Fatina Baiomy
2
Mona Wahba
3
A. Emam
4
Plant Protection Research Institute, A.R.C., Dokki, Giza, Egypt
Plant Protection Research Institute, A.R.C., Dokki, Giza, Egypt
Plant Protection Research Institute, A.R.C., Dokki, Giza, Egypt
Plant Protection Research Institute, A.R.C., Dokki, Giza, Egypt
This study was carried out to study effect of insect infestation on some internal components of some important cactus plants. So this study was carried out to study effect of insect infestation by three important insects on the internal components of three important cactus plants, Egyption mealybug, Icerya aegyptiaca (Douglas) (Margarodidae: Homoptera) on Aloe vera (Fam. Liliaceae), Agave weevil, Scyphophorus acupunctatus L. (Curculionidae: Coleoptera) on Agave americana (Fam. Agavaceae) and Daphla aphid, Aphis nerii (Boyer) (Aphididae: Homoptera) on Calotropis procera (Fam. Asclepiadaceae). And this study was carried out at two locations (governorates), El-Orman Garden (Giza Governorate) and Antoniadis Garden (Alexandria Governorate) during 2018 season.Results obtained from this study refereed to reduce the concentration of the most internal components of theses cactus plants as result to infestation by these insects. And few internal components its concentration still did not change after infestation by these insects.Statically analysis showed highly significant differences between concentration of the most internal components of the infested cactus plants compared to concentration of the same components in the control (non infested cactus plants).
https://jppp.journals.ekb.eg/article_40907_dbc07417f52a189584e3a79c1ad47b77.pdf
eng
Mansoura University, Faculty of Agriculture
Journal of Plant Protection and Pathology
2090-3677
2090-3758
2019-02-01
10
2
137
139
10.21608/jppp.2019.40909
40909
Original Article
Biological Studies and Estimation Life Table Parameters of Duranta Aphid, Aphis durantae Theobald on Duranta Plants under Fluctuated Temperature Conditions
A. Emam
1
Abla Saad
2
Marwa Abd-Allah
3
Plant Protection Research Institute, A.R.C., Dokki, Giza, Egypt
Plant Protection Research Institute, A.R.C., Dokki, Giza, Egypt
Plant Protection Research Institute, A.R.C., Dokki, Giza, Egypt
This study was carried out to study the biology and life table parameters of Duranta aphid, Aphis durantae Theobald under laboratory conditions throughout three different temperatures degrees (winter, spring and summer) during 2018 year. Data obtained showed that the spring season more suitable for A. durantae living (biology) more than winter and summer seasons, respectively. This showed clearly from life table's parameters which showed that the nymphal stages of A. durantae in spring season were less than in winter and summer, respectively. Also, life cycle, mean generation time, live span and longevity duration in spring season were less than in winter and summer seasons respectively. Lastly, the important parameter in the life table, intrinsic rate of increase (rm) in spring season was more than in winter and summer seasons, respectively.
https://jppp.journals.ekb.eg/article_40909_30058b2dcb64d7632683dd786e44ce49.pdf
eng
Mansoura University, Faculty of Agriculture
Journal of Plant Protection and Pathology
2090-3677
2090-3758
2019-02-01
10
2
141
146
10.21608/jppp.2019.40911
40911
Original Article
Population Density of the Striped Mealybug Ferrisia virgata (Ckll.) (Hemiptera: Pseudococcidae) on the Ornamental Corn Shrubs, Dracena fragrans in Relation to Biotic and Abiotic Factors in Al-Zohria Gardens, Cairo, Egypt
T. Ata
1
S. El-Awady
2
A. El-Khouly
3
M. Metwally
4
Dep. of Economic Entomology Fac. of Agric. Damietta University Cairo, Egypt.
Dep. of Plant Protection. Fac. of Agric. Al-Azhar University. Cairo. Egypt.
Dep. of Plant Protection. Fac. of Agric. Al-Azhar University. Cairo. Egypt.
Dep. of Plant Protection. Fac. of Agric. Al-Azhar University. Cairo. Egypt.
The striped mealybug Ferrisia virgata (Ckll.) (Hemiptera: Pseudococidae) attacks many host plants including shrubs and trees in Egypt. The aim of this work is to study the population densities of striped mealybug and the effect of biotic and abiotic factors on the insect population and the preferable direction for the insect stages on ornamental corn shrubs, Dracena fragrans in Al-Zohria gardens at Cairo during two successive years (2014-2015) (2015-2016). The results showed that the highest number of F. virgata insects occurred during Sep. in the first year, while it occurred during Nov. in the second year, with average (93.5 and 42.1 insect / sample) in the first and second years respectively. The general mean number of F. virgata has of 24.7 during the first year while it was 16.6 in the second year of investigation. The results showed that the insect population was highest in autumn with average (64.3 and 44.6 insect/ sample) in the first and second year respectively, whereas the lowest mean was in spring (2.7 and 1.5 insect / sample) during the two successive years. The data revealed insignificant relation between daily maximum, range and minimum of temperature and natural enemies on population activity. On the other hand the relation between the relative humidity and the population activities was highly significant positive correlation in both two years. In respect to preferable direction, results showed that the north-eastern direction was the preferable direction for the population activity in the both years of the study.
https://jppp.journals.ekb.eg/article_40911_da2b30063e17aa03914c0bf68423d8fb.pdf
the striped mealybug
population density
ornamental corn shrubs
biotic and abiotic factors
Egypt
eng
Mansoura University, Faculty of Agriculture
Journal of Plant Protection and Pathology
2090-3677
2090-3758
2019-02-01
10
2
147
153
10.21608/jppp.2019.40914
40914
Original Article
Population Density of Empoasca Spp.;Liriomyza trifolii (Burgess) and the Common Natural Enemies on Faba Bean Plants in Relation to Sowing Dates in Kafr El-Sheik Governorate
E. El-Sarand
1
E. Refaei
2
M. El-Dewy
3
Plant Protection Research Institute, Agricultural Research Center ,Giza, Egypt
Plant Protection Research Institute, Agricultural Research Center ,Giza, Egypt
Plant Protection Research Institute, Agricultural Research Center ,Giza, Egypt
Leafhoppers, Empoasca spp. (Homoptera: Cicadellidae) and The serpentine leafminer, Liriomyza trifolii (Burgess) (Diptera:Agromyzidae) are important harmful insect pests of faba bean, Vicia faba L.( Sakha 1 variety ), which causes considerable loss in yield. So, population density of these insects and the common natural enemies in relation to sowing dates were studied on faba bean during two successive growing seasons, 2016/17 and 2017/18 at Kafr EL-Sheikh Governorate. The obtained results revealed that population densities of Empoasca spp. were higher in mid- October plantation than mid- November plantation in first and second season, respectively with two peaks during both growing season. Population densities of L. trifolii larval recorded two peaks of abundance for the first and second sowing date during the two study seasons. The rate of emerging (Opius sp.) parasitoids synchronized with high parasitism. Statistical analysis cleared that the positive higher relation between rate of parasitism and population of leafminers larvae in second plantation in first season also, between rate of parasitism and emerged parasitism in second season.The population of L. trifolii in (first season) mid- November was significant than first one. The rate of parasitism as well as the emerging parasitoids was significantly higher in mid –November than mid- October (second season )Statistical analysis cleared that the population of leafhoppers; rate of parasitism and associated predatorswere insignificant differ during the two planting dates, while leafminers were significant in first seasons on contrast in the second one. On the other hand, the common predatory were significantly higher on mid-October than mad-November in the second one synchronized with the highest levels of leafhoppers and leafminers. These results could be useful in developing programs of integrated insect management in faba bean fields to reduce insecticide.
https://jppp.journals.ekb.eg/article_40914_f348d77e570296090a83f5fd1347024d.pdf
population density, parasitism
parasitoid, Opius sp
Leafhoppers, Empoasca spp
Cicadellidae
serpentine leafminer, Liriomyza trifolii
integrated and management
eng
Mansoura University, Faculty of Agriculture
Journal of Plant Protection and Pathology
2090-3677
2090-3758
2019-02-01
10
2
155
160
10.21608/jppp.2019.40916
40916
Original Article
Antibacterial and Biochemical Activities of Phenylpropenes and Monoterpenes on Phytopathogenic Bacteria
Gehan Marei
1
S. Abdelgaleil
2
Department of Plant Protection, Faculty of Agriculture, Damanhour University, Damanhour 22516, Egypt
Department of Pesticide Chemistry and Technology, Faculty of Agriculture, El-Shatby, Alexandria University, Alexandria 21545, Egypt
In the present study, six monoterpenes and twophenylpropenes were evaluated for their antibacterial effect against three phytopathogenic bacteria, Agrobacterium tumefaciens, Ralstonia solanacearum and Erwinia carotovora var. carotovora. The inhibitory effects of these compounds on polygalacturonase and dehydrogenases activities were also tested. The results revealed that trans-cinnamaldehyde, (-)-citronellal, (-)-terpinen-4-ol had the highest antibacterial activity against A. tumefaciens. Their minimum inhibitory concentration (MIC) values were 1000, 1500 and 1500 mg/l, respectively. Similarly, trans- cinnamaldehyde (MIC = 2000 mg/l), and (-)-citronellal (MIC = 2000 mg/l) were the highest activity compounds against E. carotovora var. carotovora. Moreover, (-)-citronellal caused the greatest antibacterial effect against R. solanacearum with MIC value of 1000 mg/l. Further, trans- cinnamaldehyde showed the highest inhibitory effects on polyglacturonase and dehydrogenases activities of A. tumefaciens, while (-)-citronellal represented the most potent effect of inhibition on polyglacturonase and dehydrogenases activities of E. carotovora var. carotovora and R. solanacearum.
https://jppp.journals.ekb.eg/article_40916_17fad1e39ee0e349913cf8eb429d7fe9.pdf
Phytopathogenic bacteria
Minimum inhibitory concentration
Monoterpenes
phenylpropenes
Polygalacturonase
dehydrogenases