eng
Mansoura University, Faculty of Agriculture
Journal of Plant Protection and Pathology
2090-3677
2090-3758
2018-04-01
9
4
253
259
10.21608/jppp.2018.41398
41398
Original Article
Potential Insecticidal Activity of some Medicinal Plants Essential Oils against Red Flour Beetle ,Tribolium castaneum (Herbst) (Coleoptera:Tenebrionidae).
Aisha Abdelrazik
1
A. Sallam
2
Hoda Salem
3
E. Hafez
4
E. Abdel Razik
5
Entomology Zoology Department Faculty of Science Mansoura University, Egypt.
Entomology Zoology Department Faculty of Science Mansoura University, Egypt.
Entomology Zoology Department Faculty of Science Mansoura University, Egypt
Dept. of Plant Protection and Biomolecular diagnosis, Arid Lands Cultivation Research Institute, City of Scientific Research and Technology Applications, Borg El Arab, Egypt.
Dept. of Plant Protection and Biomolecular diagnosis, Arid Lands Cultivation Research Institute, City of Scientific Research and Technology Applications, Borg El Arab, Egypt.
Tribolium castaneum is a major pest of wheat grain flour. Studies were carried out to investigate the insecticidal activity of essential oils (EOs) extracted from locally grown seven medicinal plant such as ginger )Zingiber officinale(, garlic (Allium sativum), thyme (Thymus vulgaris), clove (Syzygium aromaticum), dill (Anethum graveolens), eucalyptus (Eucalyptus globulus) , and lemon (Citrus limon) against Tribolium castaneum. In addition, Gene expression analysis for three insecticide resistance genes (Cytochrome P450 similar gene, CYP4Q4 and CYP4Q7) that mediated the detoxification mechanism, The EOs showed effective mortality of adult and larval stages of tested insect at concentration levels (6.25, 12.5 and 25) and exposure periods under laboratory conditions as compared with untreated experiments. Overall results, among essential oils A. graveolens and S. aromaticum showed highest mortality rate, followed with T. vulgaris and A. sativum while the minimum mortality rate was in E .globules against T. castaneum adult and larval stage, highest LC50 values was recorded in Anethum graveolens (LC50 at 1.5 hrs),it is note worthy that larvae more sensitive to EOs than adult in addition, Eos showed maximum expression level in T. castaneum larval stage was in Cytochrome P450 similar gene 3.38 Fold changes in mRNA, followed with CYP4Q4 and CYP4Q7 were 2.21 fold and 1.41 fold respectively as compared with reference gene (house keeping gene, β-Actin). Hence, these EOs of investigated plants may be recommended as botanical insecticide to control of stored grain insect, T. castaneum.
https://jppp.journals.ekb.eg/article_41398_c8b681373e912b97f2e4fc5f1bda121b.pdf
Medicinal plant
Essential oils
T. castaneum
mortality
Real-time-PCR
resistance genes and gene expression
eng
Mansoura University, Faculty of Agriculture
Journal of Plant Protection and Pathology
2090-3677
2090-3758
2018-04-01
9
4
261
264
10.21608/jppp.2018.41404
41404
Original Article
Biochemical Effects of Mullicicides against the Land Snails, Helicella vestalis and Theba pisana Using Sublethal Doses
Wafaa Shahawy
1
Harmful Animals Department, Plant Protection Research Institute, Agricultural Research Center, Dokki, Giza.
Land snails are serious pests in orchards, resulting in quantitative and qualitative losses in several plant species. The biochemical effects of two mullicicides; Agrinate and Biomagic were studied on two land snail species; Helicella vestalis and Theba pisana. The negative effects of sub-lethal concentration (1/4 LC50) of both mullicicides on aspartate aminotransferase, alanine aminotransferase, total lipids and total proteins were assessed. Chemical assessments were carried out at Precise Chemical Analysis Component, Plant Protection Research Institute. Results showed that the two tested compounds inhanced, in general, the activity of aspartate aminotransferase (AST) in most periods after treatment, except at the 3rd day after treatment, as both pesticides achieved a decrease in enzyme activity in H. vestalis andalso at the 10th day in T.pisana. treatment as Agrinate reduced the activity of (AST). Activity of alanine aminotransferase (ALT) was reduced in both snails, except at the 3rd day of treatment with Agrinate, while Biomagic increased (ALT) activity at the 3rd and7th days post-treatment, followed by a reduction towards the end of the test duration (two weeks). In addition, levels of total lipids (TL) and total proteins (TP) were reduced due to the application of Agrinate or Biomagic. Because enzymes, proteins and lipids are vital chemical compounds in all organisms, including land snails, it has become very necessary two assess their reactions towards mullicidal applications.
https://jppp.journals.ekb.eg/article_41404_2d35d222bcbb97e12c97edbde2769014.pdf
eng
Mansoura University, Faculty of Agriculture
Journal of Plant Protection and Pathology
2090-3677
2090-3758
2018-04-01
9
4
265
271
10.21608/jppp.2018.41405
41405
Original Article
Nematicidal Activities of Certain Animal Manures and Biopesticides against Meloidogyne incognita Infecting Cucurbit Plants under Greenhouse Conditions
A. El-Deeb
debo2012@yandex.ru
1
R. El-Ashry
2
A. El-Marzoky
3
Department of Plant Protection, Faculty of Agriculture, Zagazig University, Egypt
Department of Plant Protection, Faculty of Agriculture, Zagazig University, Egypt
Department of Plant Protection, Faculty of Agriculture, Zagazig University, Egypt.
A greenhouse experiment was carried out to evaluate the nematicidal activity of animal manures viz. chicken and goat manures, biopesticides viz. bio-zeid and nemex against Meloidogyne incognita infecting three cucurbits compared with oxamyl. Cucurbit plants namely cucumber (Cucumis sativus L.) cv. Biet Alpha, squash (Cucurbita pepo L.) cv. Escandrany and watermelon (Citrullus lanatus) cvs. Master and.Mlokywere chosen for this study. Results indicated that of five tested materials, oxamyl, chicken and goat manures were the most effective in suppressing root galling and number of egg masses of M.incognita infecting cucurbit plants. Oxamyl (69.22%), chicken (60.98%) and goat (56.60 %) manures showed better performance in reducing root galling on cucumber than did on squash. Among the two cultivars of watermelon, the effectiveness of oxamyl (70.75), chicken (52.29) and goat (49.22%) manures were more pronounced on Master than on Mloky cultivar with root galling reached 50.74, 28.36 and 23.88% respectively. On the other hand, all treatments showed significant (P ≤ 0.05) improvement in cucurbits to certain extent. The maximum percentage of increase in shoot fresh weight was recorded on cucumber (93.30; 74.76%) and watermelon cv. Mloky (92.74; 76.81%) in pots receiving oxamyl and chicken manure, respectively. In general, number of galls showing fewer and smaller gall diameter (˂ 2 mm ) was significantly decreased by oxamyl, chicken and goat manures in the three plant species. Our results showed that chicken and goat manures could be used to increase crop yield of cucurbit plants and for controlling root-knot nematode, M.incognita .
https://jppp.journals.ekb.eg/article_41405_cfb059560b73a31aab49430c9672549d.pdf
control
Meloidogyne incognita
Oxamyl
manures
biopesticides
eng
Mansoura University, Faculty of Agriculture
Journal of Plant Protection and Pathology
2090-3677
2090-3758
2018-04-01
9
4
273
276
10.21608/jppp.2018.41406
41406
Original Article
Effect of the Whey Protein on Economic Traits for Mulberry Silkworm, Bombyx mori L.
Y. Abdel-Rahman
1
Plant Protection Department, Faculty of Agriculture, Al-Azhar University, Assiut, Egypt
Prosperous silk production as well as quality of silk cocoon depend on the nutritional value of mulberry leaves and larval growth. The present study is carried out to evaluate the efficiency of whey protein on economic traits for silkworm, Bombyx mori L. Three concentrations from liquid whey protein (4, 6, and 8 %) were sprayed on mulberry leaves, also five grams from raw whey protein (powder) were used in feeding of 5th instar larvae. Control larvae were feed on untreated mulberry leaves. Result showed that, the highest mean of shell weight were (0.221, 0.216 and 0.203 g) recorded at concentration 4, 6 and 8% for liquid whey protein, respectively. The lowest mean of shell weight were (0.199 and 0.195 g) in raw whey protein and control, respectively. Highest growth index of larval of 5th instar was recorded in liquid whey protein were (5.009, 3.901 and 3.364) at concentrations 8, 4, and 6% respectively.
https://jppp.journals.ekb.eg/article_41406_ffe6c1b56bcc33950bee5eff6ecaa263.pdf
whey protein
Growth index
Cocoon characters
Mulberry silkworm
Bombyx mori
eng
Mansoura University, Faculty of Agriculture
Journal of Plant Protection and Pathology
2090-3677
2090-3758
2018-04-01
9
4
277
281
10.21608/jppp.2018.41408
41408
Original Article
Toxic and Repellent Effects of Four Plant Oils against the Red Flour Beetle, Tribolium castaneum (Herbst)
Y. Abdel-Rahman
1
M. Mahmoud
2
Plant Protection Department, Faculty of Agriculture, Al-Azhar University, Assiut, Egypt
Plant Protection Department, Faculty of Agriculture, Al-Azhar University, Assiut, Egypt
The present study was carried out to evaluate the toxic and the repellent effects of four essential plant oils Coconut oil (Cocus nucifera L.), Gingeroil (Zingiber officinale Rosc.), Jojoba oil(Simmondsia chinensis L.) and spearmint oil(Mentha spicata L.) against the adult of red flour beetle. Three concentrations of each plant oils were used, 0.5, 1 and 2%. The LC50, LC90 values were also calculated using Probit analysis technique. LC50, LC90 values of coconut, ginger, jojoba and spearmint oils after 24, 48, 72 and 96 hrs intervals were determined. Results revealed that ginger oil was more effective against Tribolium castaneum. Data of 96 hrs showed that ginger still the most effective oil against the pest (LC50 = 0.37 and LC90 = 0.86%). The LC50 values were 0.56, 0.66 and 1.17% and LC90 values were1.09, 1.19 and 2.76% for coconut, spearmint and jojoba, respectively. Repellency test was conducted at 2% concentration for each oil. Data were recorded at 1, 3, 6, 12 and 24 hrs after insect release. The results showed that the highest repellent rate (45.08) was recorded for ginger and spearmint oil, while the lowest repellent rate (15.01) was recorded for jojoba oil after 24 hrs.
https://jppp.journals.ekb.eg/article_41408_535002d32ee1321b914925113ab21ad5.pdf
toxicity
repellency
Tribolium castaneum
plant oils