Mansoura University, Faculty of Agriculture
Journal of Plant Protection and Pathology
2090-3677
2090-3758
8
5
2017
05
01
In vitro Evaluation of Certain Fungicides Against Botrytis cinerea Isolates the Causal Pathogen of Gray Mold Disease on Tomato
195
200
EN
N.
D.
Dib
Ministry of Higher Education.
T.
G.
Abdel Rahman
Plant Pathol. Res. Inst., ARC, Giza, Egypt.
A.
M. A.
Ashour
Plant Pathol. Dept., Fac. Agric., Cairo Univ.
H.
M. A.
Badawy
Economic Entomol. & Pest. Dept., Fac. Agric., Cairo Univ.
10.21608/jppp.2017.46199
Efficiency of fungicides; Teldor <strong>50%SC, </strong>Nowton 50% SC, Toledo 43% EC, Kenzo 50% WG and Suntop El-nasr 70% WP against tomato gray mold disease was conducted <em>In vitro</em> tests. The sensitivity of <em>Botrytis cinerea</em> isolates to tested fungicides differed significantly according to their aggressiveness. The lowest pathogenicity isolate BcI-3 ranked as the most susceptible isolate, while the highest pathogenicity isolate BcB-2 was the least susceptible isolate to the tested fungicides. Medium effective concentrations (EC<sub>50</sub>) of the tested fungicides indicated that Teldor <strong>50%SC</strong> was the superior fungicides against mycelial growth of the all tested isolates, while Suntop El-nasr 70% WP was the inferior one. The EC<sub>50</sub> values of the tested fungicides for mycelial growth of the least aggressive isolate (BcI-3) were less than that of the other tested isolates. Under greenhouse condition, all tested fungicides were control gray mold disease on tomato. Data revealed that when tested fungicides treated as curative or protective, were effective in reducing the disease severity of gray mold produced from the most and least aggressive isolates. Tested fungicides Teldor <strong>50% SC</strong> and Toledo 43% EC gave excellent control of gray mold disease when applied as protective or curative. All tested fungicides, expect Suntop El-nasr 70% WP, were effective in controlling gray mold as curative treatement.
Tomato gray mold disease,fungicides,mycelial growth,greenhouse
https://jppp.journals.ekb.eg/article_46199.html
https://jppp.journals.ekb.eg/article_46199_0eb99644261cf0c273ef135700d515e6.pdf
Mansoura University, Faculty of Agriculture
Journal of Plant Protection and Pathology
2090-3677
2090-3758
8
5
2017
05
01
In vitro and In vivo Evaluation of Certain Fungicides Against Phytophthora Infestans (Mont.) The Causal Pathogen of Late Blight Disease on Tomato
201
208
EN
N.
D.
Dib
Ministry of Higher Education.
T.
G.
Abdel Rahman
Plant Pathol. Res. Inst., ARC, Giza, Egypt
A.
M. A.
Ashour
Plant Pathol. Dept., Fac. Agric., Cairo Univ.
H.
M. A.
Badawy
Economic Entomol. & Pest. Dept., Fac. Agric., Cairo Univ.
10.21608/jppp.2017.46201
Efficiency of fungicides; Shirlan 50% SC, Ranman 40% SC, Revus 25% SC, Leimay 20% SC, Vegeclean 15% WG and Evito 48% SC against tomato late blight disease was conducted <em>In vitro</em> and <em>In vivo</em> tests. The variation in the aggressiveness of the two isolates of <em>P. infestans</em> could be clearly differentiated to most aggressive one (PhK-2) and least aggressive (PhK-1). Medium effective concentrations (EC<sub>50</sub>) of the candidate fungicides indicated that Shirlan 50% SC was the superior fungicides against mycelial growth of PhK-1 and PhK-2 isolates. Revus 25% SC and Ranman 40% SC were less efficiency against mycelial growth of PhK-1 and PhK-2 isolates, respectively. The tested fungicides gave excellent control of tomato late blight disease under greenhouse conditions especially Evito 48% SC and vegeclean 15% WG when applied as protective or curative. The candidate fungicides were used separately at their recommended rates of application against late blight disease on tomato plants during the season of 2015-2016 in two different locations (Fayoum and Sharkia Governorates). In Sharkia Governorate, all the candidate fungicides gave excellent control of late blight disease, which their efficiency ranged between 80.03 and 85.93% except Revus 25% SC gave 63.67% only. Efficiency of Leimay 20% SC and Shirlan fungicides against late blight disease was increased from 63.73 and 65.61% in Fayoum trial to 80.03 and 80.69% in Sharkia trial, respectively. Efficacy percentages of tested fungicides were highly correlated with area under the disease progress curve (AUDPC) values. All candidate fungicides when treated as curative were effective in controlling late blight disease except Revus 25% SC.
Tomato late blight disease,fungicides,mycelial growth,greenhouse,field trials
https://jppp.journals.ekb.eg/article_46201.html
https://jppp.journals.ekb.eg/article_46201_94c448ba6fb2d9df0a91a51f3f76001c.pdf
Mansoura University, Faculty of Agriculture
Journal of Plant Protection and Pathology
2090-3677
2090-3758
8
5
2017
05
01
Field Evaluation of Some Insecticides on some Cowpea Pests and Their Side Effect on Associated Predator (Chrysoperla carnea, steph.).
209
214
EN
Moshera
Ahmed
Plant protection Res. Institute, ARC, Dokki, Giza, Egypt
A.
A. A.
Said
Pesticides Dept. Faculty of Agric., Mansoura Univ., Egypt.
A.
A.
Saleh
Pesticides Dept. Faculty of Agric., Mansoura Univ., Egypt.
W.
G. T.
Gatwary
Plant protection Res. Institute, ARC, Dokki, Giza, Egypt.
Laila
R. A.
El-Gohary
Pesticides Dept. Faculty of Agric., Mansoura Univ., Egypt.
10.21608/jppp.2017.46207
This study was carried out to field evaluate the efficacy of nine insecticides namely chlorpyrifos, emamectin benzoate, thiacloprid, acetamiprid, imidacloprid, indoxacarb, lufenuron, <em>Metahrizum ansoplia,</em> and Kz oil at the recommended rate against aphid, <em>Aphis craccivora </em>Koch; whitefly, <em>Bemisia tabaci</em> (Genn.); the green bug, <em>Nizara viridula</em> L; the cotton leafworm, <em>Spodoptera littoralis</em> (Boisd.) and its predator Green lacewing, <em>C. carnea</em> (Stephens) under field conditions during 2013 and 2014 Cowpea seasons. Data showed that emamectin benzoate and chlorpyrifos were the highest effect in initial kill, residual effect and mean reduction on <em>A. craccivora</em>, <em>B. tabaci</em>, <em>N. viridula</em> and <em>S. littoralis</em>; while Kz oil and <em>Metahrizum ansoplia</em> were the lowest effect, during 2013 and 2014 seasons.Imidacloprid, thiacloprid and acetamiprid recorded moderate effect in initial kill, residual effect and mean reduction on<em> B. tabaci</em> and <em>N. viridula</em> while they recorded low effect on <em>S. littoralis</em>. Indoxacarb caused the moderate effect against <em>S. littoralis</em> while it recorded low effect on <em>B. tabaci</em>.On the other hand lufenuron showed high effect on <em>A. craccivora </em>and <em>S. littoralis</em> andrecorded moderate effect on the <em>N. viridula</em>; while it recorded low effect on <em>B. tabaci</em>.The most toxic insecticides to <em>C. carnea</em> were chlorpyrifos, lufenuron and emamectin benzoate while Kz oil and <em>Metahrizum ansoplia </em>were toxic less to the predator on the studied two seasons.
Cowpea pests (piercing sucking pests,cotton leafworm and predator,Chrysoperla carnea) – insecticides – initial kill – residual effect – reduction percentage
https://jppp.journals.ekb.eg/article_46207.html
https://jppp.journals.ekb.eg/article_46207_72f5e73559a49eeecc4fc4ba3d9772c4.pdf
Mansoura University, Faculty of Agriculture
Journal of Plant Protection and Pathology
2090-3677
2090-3758
8
5
2017
05
01
The Effect of Some Bio-Insecticides on the Cotton Leaf Worm Spodoptera littoralis (Boisd.) Larvae
215
218
EN
Aziza
A. M.
Darwish
Faculty of Science, Mansoura University
G.
A.
El-Mezayyen
Plant Protection Research Institute, Agriculture research center, Dokki, Giz
M.
A.
El-Magd
Department of Anatomy, Faculty of Veterinary Medicine, Kafrelsheikh University
Suzan
M. S.
Badr
Plant Protection Research Institute, Agriculture research center, Dokki, Giz
10.21608/jppp.2017.46210
This study was conducted to investigate the effect of LC<sub>50</sub>concentration of three commercial bio insecticides; viruset,<em> spodopetra littoralis</em> nuclear polyhedrosis virus, protecto <em>Bacillus thuringensis</em>var. <em>kurstaki</em>and their mixture profect on the protein profile and enzyme activity of three carbohydrate digestive enzymes (amylase, invertase and trehalase).The digestive enzymes were determined in 6<sup>th </sup>larva linstar after treatment of 3<sup>rd</sup> larval instar with the LC <sub>50 </sub>concentration. Results showed that amylase activity was relatively un affected in treated larvae with viruset and protecto,but there was asignificant decrease when treated with profect compound.On the other hand the activity trehalase was significantly decreased in all samples. Also protein profile of the treated and untreated larvae was studied by SDS –PAGE technique. Results showed that the protein pattern of larvae treated with the profect compound leads to inhibition and appearance of some bands followed by protecto compound, while viruset has a slight change in the pattern. Thus, our findings indicated that among the three tested compounds, profect compound showed a highest significant effect on both protein profile and enzyme activity of the larvae.
https://jppp.journals.ekb.eg/article_46210.html
https://jppp.journals.ekb.eg/article_46210_10dbfff6d10e0fac743d4a22acfc6161.pdf
Mansoura University, Faculty of Agriculture
Journal of Plant Protection and Pathology
2090-3677
2090-3758
8
5
2017
05
01
Inhibitory Effects of Methoxyfenozide on Reproductive Organs of Cotton Leafworm, Spodoptera Littoralis (Boisd.)
219
227
EN
Hend
M.
Sabry
Plant Protection Research Institute, Agricultural Research Center, Dokki, Giza, Egypt
Hala
M. I.
Mead
Plant Protection Research Institute, Agricultural Research Center, Dokki, Giza, Egypt
Karima
S.
Khater
Faculty of Science , Zoology Department, Zagazig University, Egypt
10.21608/jppp.2017.46211
Current research has focused on the inhibitory effects on the reproductive organs of cotton leafworm, <em>Spodoptera littoralis</em> resulting from 4<sup>th</sup> instar larvae treated with sublethal concentration (LC<sub>25</sub>) of methoxyfenozide. Results showed elongation in the immature stages and longevity of adults than control, as well as reduce pupal weight, oviposition period, fecundity and hatchability percentages. Anatomical structures of virgin moths surviving from larvae treated with LC<sub>25</sub>of methoxyfenozide showed general atrophy and abnormal features for ovaries and testes than thatof untreatedones. Histological disruptions were recorded in ovaries in vitellogenesis and post vitellogensis stages as deformation in follicular shape, clumping of the chromatin material in nurse cells and disorganization of yolk and appearance of vacuoles between yolk and chorion. While in treated testes the testicular wall disappeared and vacuoles between follicles take place. Methoxyfenozide caused alterations in the amounts of both total soluble protein and total lipids and also in the activities of acid and alkaline phosphatases and phenol oxidase in the ovaries and testes of the resulted adults as compared to control adults.
Spodoptera littoralis,toxic,histochemical,Enzymes,Methoxyfenozide
https://jppp.journals.ekb.eg/article_46211.html
https://jppp.journals.ekb.eg/article_46211_2bf2edbd1c41101f50348779f4d69834.pdf