Mansoura University, Faculty of Agriculture
Journal of Plant Protection and Pathology
2090-3677
2090-3758
10
8
2019
08
01
Comparative Studies on the Free Amino Acid Composition in the Total Body Homogenate of some Lepidopterous and Neuropterous Insects دراسة لمقارنة محتوى الأحماض الأمينية لبعض حشرات رتبتي حرشفية وشبکية الأجنحة
391
395
EN
رانيا
الشناوي
قسم بحوث ديدان اللوز - معهد بحوث وقاية النباتات – مرکز البحوث الزرعية
دينا
أحمد
قسم بحوث ديدان اللوز - معهد بحوث وقاية النباتات – مرکز البحوث الزرعية
منال
الشرقاوى
قسم بحوث ديدان اللوز - معهد بحوث وقاية النباتات – مرکز البحوث الزرعية
10.21608/jppp.2019.58171
Three different insect species belonging to three families [i.e. <em>P. gossypiella </em>(PBW)<em>, S. littoralis </em>(CLW)and<em> C. carnea </em>(APL)] were studied for their amino acid composition. Seventeen free amino acids (FAA) figured out in the total body homogenate of the tested insects. The essential AA were threonine (THR), valine (VAL), methionine (MET), isoleucine (ILE), leucine (LEU), phenylalanine (PHE), lysine (LYS). Semi-essential AA were histidine (HIS), arginine (ARG), cysteine (CYS), and Non-essential were aspartic acid (ASP), serine (SER), glutamic acid (GLU), glycine (GLY), alanine (ALA), tyrosine (TYR), and proline (PRO). Generally, results showed that non-essential AA were the most predominant fraction of FAA, following by essential then semi-essential AA, and the PBW had the highest average of AA (1.89, 1.32 & 2.41 for essential, semi-essential & non-essential AA, respectively), followed by APL (1.78, 1.22 & 2.34, respectively), then CLW (1.66, 1.16 & 2.17, respectively). In details, from the results it could be grouped AA in 3 groups as follows: 1- A high AA concentration (more than 3 mg/100mg). 2- A moderate AA concentration (more than 2 mg/100mg). 3- A low AA concentration (less than 2 mg/100mg).The results showed that the PBW characterized by a high AA concentration of ASP (3.19 mg/100mg) and GLU (4.24 mg/100mg), while CLW and APL had only a high AA concentration of GLU (3.98 & 5.24 mg/100mg, respectively), and a moderate concentration of ASP (2.46 & 2.90 mg/100mg, respectively). In addition, GLU had a highest concentration in APL. The obtained results indicated that glutamic acid and aspartic acid (non-essential AA) were the most predominant FAA. For moderate AA, PBW characterized by an amino acids VAL and TYR (2.28 mg/100mg; 17.26% & 2.53 mg/100mg; 13.88%, respectively). In contrast, CLW and APL had a low VAL and TYR concentration (1.73 & 1.78 mg/100mg; 14.91 & 14.25% & 1.63 & 1.87 mg/100mg; 9.96 & 10.61% for VAL & TYR, respectively). Also, ALA had a moderate concentration in PBW (2.28 mg/100mg) and CLW (2.50 mg/100mg), while had a low concentration in APL (1.83 mg/100mg). In addition, CYS had a least concentration of AA (0.55, 0.59 and 0.57 mg/100mg for PBW, CLW and APL, respectively.
https://jppp.journals.ekb.eg/article_58171.html
https://jppp.journals.ekb.eg/article_58171_a60bb63eb1407f8af013debf486c69f5.pdf
Mansoura University, Faculty of Agriculture
Journal of Plant Protection and Pathology
2090-3677
2090-3758
10
8
2019
08
01
Method Development and Validation of Carbendazim Fungicide in Cucumber تطوير واعتماد طريقة لتحليل المبيد الفطرى الکاربندازيم فى الخيار
397
400
EN
علاء
مسعود
خزيمى
قسم وقاية النبات - کلية الزراعة - جامعة دمنھور - مصر
محمد
فتحی عبد الرحمن
رمضان
مرکزالبحوث الزراعية - المعمل المرکزي للمبيدات - قسم بحوث تحليل المبيدات - الدقي - الجيزة – مصر
10.21608/jppp.2019.58178
Evaluation of pesticide residues was carried out in cucumber to determine the residues of carbendazim which collected from different local markets in Egypt. During a 14-day, treated cucumbers samples were gathered and analyzed to determine the carbendazim residue quantity. A QuEChERS sample preparation has been applied with liquid chromatography provided with mass spectrometry (LC/MS-MS) to evaluate residual of tested fungicide. Two transitions ions was obtained from multiple reaction monitoring (MRM) after that higher sensitivity transition used for quatification but the lower sensitivity transitions used to confirmation analysis of carbendazin. The method was validated by setting performance parameters such as linearity, precision, recovery, limits of detection (LOD) (0.45 µg Kg<sup>1</sup>) and limits of quantification (LOQ) (0.9 µg Kg<sup>1</sup>). Good correlation coefficient R<sup>2</sup> 0,9992 in pure solvent curve and R<sup>2</sup> 1.000 for matrix-matched curve, which given the elevated calibration curve quality. The carbendazin recoveries founded in fresh cucumber samples of 10 replicates were found to be in the range between 119.11 to 124.45% (% RSD > 3.5) for two fortification levels (0.010 and 0.070 mgKg<sup>1</sup>). The intra-day repeatability RSD value was 3.26%, while the inter-day repeatability RSD value was 5.27%. Matrix effect of carbendazim in cucumber was evaluated and the results showed that carbendazim had a soft matrix effect (6.53).Samples of tested fungicides showed distinct contamination levels up to a certain time period (7 days), after which no residues were identified between 11-14 days.
Pesticide residue,Ultra Performance Liquid Chromatography,MS Detector,Carbendazim
https://jppp.journals.ekb.eg/article_58178.html
https://jppp.journals.ekb.eg/article_58178_f7da563cec5729760a90f313434fbec5.pdf
Mansoura University, Faculty of Agriculture
Journal of Plant Protection and Pathology
2090-3677
2090-3758
10
8
2019
08
01
Evaluation of Ethephon Application on Bollworms, Pectinophora gossvpiella (Saund.) and Earias insulana (Bois.) Infestation and Boll Opening in Cotton Fields تقييم استخدام الإثيفون علي الإصابة بديدان اللوز وتفتيح لوز القطن في حقول القطن
401
402
EN
عبد البديع
عبد الحمید
غانم
قسم الحشرات الاقتصادية- کلية الزراعة – جامعة المنصورة
جمال
على
المزين
معهد بحوث وقاية النباتات – مرکز البحوث الزراعية – دقى - جيزة
عبد الخالق
السید عبد الرازق
حاتم
معهد بحوث وقاية النباتات – مرکز البحوث الزراعية – دقى - جيزة
10.21608/jppp.2019.58182
The present study was conducted at Sakha Agricultural Research Station, Kafr El—Sheikh governorate, Egypt during 2016 and 2017 late cotton seasons. Results showed that Ethephon (plant growth regulator) treatment gave the highest reduction percentage, 56.68 and 37.60% of pink bollworm infestation, <em>Pectinophora gossvpiella</em> (Saund.) with 3 cm3/L, while they were 50.66 and 43.75% of spiny bollworm infestation, <em>Earias insulana</em> (Bois.) with 1.5 cm<sup>3</sup>/L in 20l6 and 2017 cotton growing seasons, respectively. On the other hand, concentration of Ethephon, 2.5 cm<sup>3</sup>/L gave the highest increasing of percentages of mature boll opening recording 36.68 arid 22.57% in the two tested seasons respectively, It could be concluded that using Ethephon with 3.0 cm<sup>3</sup>/L to accelerate mature boll opening in late cotton season.
Ethephon,bollworms,Pectinophora gossypiella,Earias insulana,boll opening
https://jppp.journals.ekb.eg/article_58182.html
https://jppp.journals.ekb.eg/article_58182_274b14ad0c8665d59f09427715c0feb3.pdf
Mansoura University, Faculty of Agriculture
Journal of Plant Protection and Pathology
2090-3677
2090-3758
10
8
2019
08
01
Predication The Cotton Leafworm, Spodoptera littoralis (Boisd.) Field Geuerations As Influenced by Heat Unit Accumulation التنبؤ بالأجيال الحقلية لدودة ورق القطن، باستخدام الوحدات الحرارية التراکمية
403
406
EN
جمال
على
المزين
معهد بحوث وقاية النباتات – مرکز البحوث الزراعية – دقى - جيزة
عبد البديع
عبد الحمید
غانم
قسم الحشرات الاقتصادية- کلية الزراعة – جامعة المنصورة.
عبد الخالق
السید عبد الرازق
حاتم
معهد بحوث وقاية النباتات – مرکز البحوث الزراعية – دقى - جيزة
10.21608/jppp.2019.58185
The present study was conducted at the experimental farm, Sakha Research station, Kafr El –Sheikh region under field conditions during the seasons of 2016 and 2017. Results indicated that the population of the cotton leafworm moths. <em>Spodoptera littoralis</em> (Boisd.) had seven peaks starting from the 3<sup>rd</sup> week of May until the 1<sup>st</sup> week of September during the two studied seasons. The highest number of moths was recorded during the end of June which being 8023.2 and 2063.2 moth /trap/ 30 days and 60 days after cotton planting in both seasons, respectively. The predicted peaks of generations could be detected when the accumulated thermal units recorded 525.42 DD's. The predicted peaks for the detected four generations varied from +1 to +8 days from the observed peaks. For better prediction of the cotton leafworm the period between the observed and expected peaks should be positive and as short as possible when early preparation of control materials are of great important, consequently, it could be helpful when IPM tactics are considered.
Cotton leaf worm,Spodoptera littoralis,peaks,predicting,generations,thermal units accumulations
https://jppp.journals.ekb.eg/article_58185.html
https://jppp.journals.ekb.eg/article_58185_534d493cd3e8ded091a76ed6216b5857.pdf
Mansoura University, Faculty of Agriculture
Journal of Plant Protection and Pathology
2090-3677
2090-3758
10
8
2019
08
01
Seasonal Occurrence of Parlatoria ziziphi (Lucas) and Its Parasitoid in Relation with some Climatic Factors and Chemical Components on Navel Orange Trees التواجد الموسمى لحشرة parlatoria ziziphi (Lucas) وطفيلها الحشرة فى علاقه مع بعض العوامل المناخية والمکونات الکيمائية على أشجار البرتقال أبو سرة
407
413
EN
حسن
احمد
نبيل
معهد بحوث وقاية النباتات- مرکز البحوث الزراعية – الدقى – الجيزة – مصر
علا
ابراهیم محمد
حجاب
قسم وقاية النبات – کلية الزراعة – جامعة الزقازيق – مصر
محمد
على مرسى
حجاب
قسم وقاية النبات – کلية الزراعة – جامعة الزقازيق – مصر
10.21608/jppp.2019.58186
The black parlatoria scale insect, <em>Parlatoria ziziph</em>i (Lucas) is one of the most important armored scale insect infesting citrus trees. This investigation was conducted at Inshas El-Raml district, Sharkia Governorate throughout the two seasons 2016-2017 and 2017-2018 to study some ecological aspects of this scale insect on navel orange trees. This study showed that the seasonal activity of this insect was more abundant in the first season than in the second one, due to the relation with its associated parasitoid. The <em>P. ziziphi</em> had three generations and two peaks of the total alive stages during winter and autumn seasons throughout the two years of investigation. Also, the increase or decline of populations was correlated with some climate factors as temperature, relative humidity and light intensity and also influenced by some chemical components in navel orange trees. These results can be beneficial when developing an integrated program for control this pest without the use of pesticides that may pollute the environment.
Parlatoria ziziphi,seasonal fluctuation,parasitoid,chemical components,navel orange trees
https://jppp.journals.ekb.eg/article_58186.html
https://jppp.journals.ekb.eg/article_58186_14b98ff4cba27cfcca7e0030566db5db.pdf
Mansoura University, Faculty of Agriculture
Journal of Plant Protection and Pathology
2090-3677
2090-3758
10
8
2019
08
01
Effect of Compost Alone or in Combination with some Substances Against White-Rot Disease of Onion. تأثير الکمبوست منفردًا أو مضافاً إليه بعض المواد ضد مرض العفن الأبيض فى البصل
415
421
EN
صابر
محمد
مرسى
معهد بحوث أمراض النباتات – مرکز البحوث الزراعية – الجيزة
عزة
رسمى
عمارة
المعمل المرکزي للمبيدات – مرکز البحوث الزراعية – الجيزة
10.21608/jppp.2019.58191
This study was carried out to control the onion white-rot disease by using compost alone or in combination with some other treatments such as Humic acid, sulfer, <em>Trichoderma viride</em> and gool herbicide. Six isolates of <em>Seclerotium cepivorum</em> (the causal fungus of the onion white – rot disease) were tested in this experiments. Isolate No. 1 was the most aggressive as it incited white – rot disease on three onion cultivars (Giza 20, Giza 6 and Italy). The soil application with compost which added at planting gave the lowest mean disease incidence under greenhouse conditions. However compost application alone or in combination with other amendments on infection with <em>Sclerotiomum cepivorum</em> (isolate Sc<sub>1</sub>) on onion (cvs. Giza 20 and Giza 6) significant decreased in the disease incidence(percentage of infection) as compared with the infected untreated control. Treatments of compost with gool was the most effective treatment and reduced percentage of infection with white rot on Cv. Giza 20 and Cv. Giza 6, to 3.9% and 6.95% compared to 44.6% and 53.75% for the untreated control for both Cvs. respectively, with 89.1% mean disease reduction under field conditions. Investigating of the chemical characteristics associated with onion tolerance to <em>S.</em> <em>cepivorum</em> reveald that he total phenolic content in most tolerant non-inoculated cv. Italy was obviously higher than that the most susceptible cv. Giza 20. being 9.8 mg/g. f.w. and 10.9 mg/g.f.w, respectively. Also, planting of onion plants in soil infested with S.<em>cepivorum</em> isolates significantly increased mean total phenolic content in bulbs reach 17.32 mg/g. f.w. in the most resistant cv. Italy of onion compared to the susceptible cv. Giza 20 reached to 13.8 mg/g.f.w.. Also, the application of compost alone or in combination with other substances significantly increased shoot length, number of leaves / plant and yield.
https://jppp.journals.ekb.eg/article_58191.html
https://jppp.journals.ekb.eg/article_58191_0601693d09fa3298b6b2f4cb4e84b256.pdf