Mansoura University, Faculty of AgricultureJournal of Plant Protection and Pathology2090-367711420200401Molecular Identification of some Powdery Mildew Resistance Genes in Ten Egyptian Durum Wheat Cultivars2052098759210.21608/jppp.2020.87592ENMona ElsayedWheat Diseases Research Dept., Plant Pathology Research Institute, A.R.C., 12619.0000-0003-2419-0526A. F.ElkotWheat Research Dept., Field Crops Research Institute, A.R.C., 12619.Journal Article20200507Powdery mildew, caused by <em>Blumeria graminis</em> f. sp<em>. tritici</em>, is one of the most damaging foliardiseases of wheat world-wide. Nineteen powdery mildew differential monogenic lines (<em>Pm</em>) and ten durum wheat cultivars were evaluated for powdery mildew reaction at the seedling stage in a control-conditioned glasshouse and at adult stage under field conditions during 2018/2019 and 2019/2020 growing seasons in Gemmeiza Agriculture Research Station, ARC, Egypt. At seedling stage,<em> Pm</em>13,<em> Pm</em>24, <em>Pm</em>35, <em>Pm</em>36 and <em>Pm</em>37 were completely effective against 78 tested isolates of powdery mildew followed by <em>Pm</em>16, <em>Pm</em>32, <em>Pm</em>34, <em>Pm</em>29 and <em>Pm</em>43 according to their descending order. At adult stage, all the <em>Pm</em> genes were resistant except <em>Pm</em>8 and <em>Pm</em>9, which showed susceptibility to the disease. Although, the durum wheat cultivars were susceptible to powdery mildew isolates at seedling stage, they ranged from intermediate resistant to resistant at the adult stage. To confirm the presence of resistant genes in 10 Egyptian durum wheat cultivars, five specific molecular markers i.e.<em> KSUG53</em>, <em>Xgwm</em>337, <em>Xcfd</em>7, <em>Bj</em>261635, and <em>Xgwm</em>332 linked to <em>Pm</em>13, <em>Pm</em>24., <em>Pm</em>35., <em>Pm</em>36 and <em>Pm</em>37resistance genes were selected. The linked markers used in this study assured the presence of <em>Pm</em>13, <em>Pm</em>36 and <em>Pm</em>37 in all tested durum cultivars. However, <em>Pm</em>35 was present in BeniSweif1, BeniSweif3, BeniSweif5 and BeniSweif6. Moreover, data showed<em> that Pm</em>24 was absent in all tested cultivars.https://jppp.journals.ekb.eg/article_87592_1a60d51342658be020b9a1976acfffa7.pdfMansoura University, Faculty of AgricultureJournal of Plant Protection and Pathology2090-367711420200401Toxicity of the Locally Formulated Diesel Oil Alone and Mixed with Botanical Synthetic Materials against Mealybug Ferrisia virgate (Cockerell)2112149600610.21608/jppp.2020.96006ENM. A.EskanderCentral Agric. Pesticides Lab. (CAPL), Agric. Res. Center (ARC), Dokki, Giza, Egypt.Fatma A.MoharumPlant Prot. Res. Inst., Agric. Res. Center, Dokki, Giza, Egypt.Sanaa A. M.Abd El-MageedPlant Prot. Res. Inst., Agric. Res. Center, Dokki, Giza, Egypt.Journal Article20200615Physico-chemical properties of crude diesel oil and botanical synthetic materials (thymol and camphor) were studied and prepared as emulsifiable concentrate formulation (EC). Prepared formulations passed successfully specified testes, then their toxicity were investigated against different stages of mealybug<em> Ferrisia virgata</em> (Cockerell) under laboratory conditions. Results indicated that all tested formulations showed high toxicity against all tested insect stages. The mixing, thymol or camphor with diesel oil increased toxicity of oil against mealybug <em>F. virgate</em>, whereas mixture, thymol with diesel oil was more effective than camphor with diesel oil. LC<sub>50</sub> values after two days of application were 4.02, 4.51, 4.86 and 5.33 ml/l for diesel oil, while it were 0.17, 0.17, 0.25, and 0.25ml/l for mixture of oil and thymol, and 0.88, 0.99, 1.12, and 1.24 ml/l for mixture of diesel oil and camphor for first, second, third and adult stages of <em>F. virgate</em> respectively. While LC<sub>50</sub> values after three days of application were: 1.43, 1.49, 1.61 and 1.77 ml/l for diesel oil, while it were 0.03, 0.03, 0.08 and 0.11 ml/l for mixture of oil with thymol, and 0.14, 0.24, 0.21 and 0.16 ml/l for mixture of oil with camphor for 1<sup>st</sup>, 2<sup>nd</sup>, 3<sup>rd</sup> and adult of <em>F. virgate</em> respectively. Results showed, efficacy of tested materials increased by increasing concentration and period after application and mixing botanical synthetic materials with diesel oil increased toxicity against all tested stages; moreover, mixtures could be used for controlling mealybug in medical, aromatic and ornamental plants after conducting more semi-field and field experiments. https://jppp.journals.ekb.eg/article_96006_2956737c41d5de5783c58ee608344a75.pdfMansoura University, Faculty of AgricultureJournal of Plant Protection and Pathology2090-367711420200401Some Astigmated, Prostigmated and Cryptostigmated Mites Inhabiting some Stored Products at El-Sharqia Governorate, Egypt2152199600710.21608/jppp.2020.96007ENM. E. G.GadDepartment of Zoology and Entomology, Faculty of Science (Cairo), Al–Azhar University.A. M.MetwallyDepartment of Agricultural Zoology and Nematology, Faculty of Agriculture (Cairo), Al –Azhar University.A. S. M.BreamDepartment of Zoology and Entomology, Faculty of Science (Cairo), Al–Azhar University.Journal Article20200615This work was conducted to survey the mite species associated with stored food stuff during the two successive years (2018 and 2019), in El-Sharqia Governorate. The obtained results indicated that thirty eight mite species were found in twenty four different stored products, as follows: wheat grain, wheat flour, wheat bran, rice grain, maize grain, garlic, hay, onion, pea, cumin, corn, pea, bread bean, animal feed, poultry feed, carrot, Egyptian clover, straw, okra seeds, grass, watercress, potato, house dust and barley were collected from El-Sharqia governorate, Egypt. The collected mites were found belonging to three suborders and 15 families in orderAcariformes. Suborder Acaridida (Astigmata) included 21 species belong to 6 families; suborder Actinedida (Prostigmata) included 12 species of 7 families, while suborder Oribatida (Cryptostigmata) included only five species of two families. https://jppp.journals.ekb.eg/article_96007_76f106ca1cd2bb0f02d14cdda04332db.pdfMansoura University, Faculty of AgricultureJournal of Plant Protection and Pathology2090-367711420200401Toxicity of Certain Insecticides on Tuta absoluta (Lepidoptera: Gelechiidae) with Relation to Carbohydrasis and Phosphatasis Enzyme2212249600810.21608/jppp.2020.96008ENHeba A. A.Al-GhnamDepartment of Pest Physiology, Plant Protection Res Inst., ARC, Dokki, Giza, Egypt.S. M.Abd El NabyPlant Protection Research Institute (PPRI), ARC, Dokki, GizaJournal Article20200615The present work is the studying the toxicities of three insecticides from different groups indoxacarb, emamectin benzoate and methoxyfenozide on <em>Tuta absoluta. </em>collected from field.The obtained result showed the toxicity of three insecticides against the third larvae instar of <em>T.absoluta</em>, the <sub> </sub>values of LC<sub>50 </sub>for methoxyfenzoid, indoxacarb and emmamectin benzoate (50.77 ppm. , 2.33 ppm. and 0.022 ppm.), respectively. Emmamectin benzoate and indoxacarb are more effective methoxyfenzoid. The biochemical result showed high increase in activity enzyme trehalase and Acid phosphatase (17.93% , 7.83%), respectively and slight increase in invertase and amylase activities, (2.51%, and 0.039%), respectively, when treated by Indoxicarb comparing with the other two insecticide.https://jppp.journals.ekb.eg/article_96008_38e36e5dff9ccd44c7eeb533f692ac61.pdfMansoura University, Faculty of AgricultureJournal of Plant Protection and Pathology2090-367711420200401Comparative Insecticidal Activity of Three Forms of Silica Nanoparticles on some Main Stored Product Insects2252309600910.21608/jppp.2020.96009ENAbeer A.SalemPlant Protection Research Institute, Agricultural Research Center, Dokki, Egypt.Journal Article20200615The control of stored grain pests requires effective materials that are save for humans and environment, low cost, available and easy to obtain and do not need trained labor. Therefore, the current study suggested testing three silica forms in the form of nanoparticles, these were Aerosil 200, chemical and bio-silica for controlling <em>Callosobrochus macula</em><em>tus</em> F. (Col., Bruchidae)<em>, Rhythopertha dominica </em>fab, and<em> Tribolium confusum </em>(Jacquelin du Val)<em>. </em>The obtained results showed that the percent mortality increased with the increasing of concentration and exposure period. Moreover, all the tested materials of nanoparticles had detrimental induction on mortality and offspring of the three tested insects. In addition the adults of <em>C</em>.<em>maculatus</em> were more susceptible followed by <em>R</em>.<em>dominica</em> and <em>T</em>.<em>confusum</em> with the three tested materials at the all concentrations used. Data obtained revealed that the activity of chemical silica had the first rank followed by Aerosil 200 and bio-silica where the LC50 of chemical silica ranged between 0.14-1.54 one day posttreatment with significant distinction compared to Aerosil 200 (0.15-2.65) and bio-silica (2.43-7.35)g/kg grain. The findings obtained showed that the three forms of silica had deterrent action on the all tested immature stages at the all levels of concentrations. Also results clarified the egg stage was the most susceptible compared to larvae and pupae ones. The all treatments reduced the emerged adults and the losses of grain weight. Consequently the present study explained that the three used forms of silica are promising materials for controlling the tested insects as safe alternatives to the synthetic insecticides.https://jppp.journals.ekb.eg/article_96009_65aca73a90c83f9998b2360a08c551fa.pdfMansoura University, Faculty of AgricultureJournal of Plant Protection and Pathology2090-367711420200401Influence of Emamectin Benzoate ; Emamectin Nanoformulations and some Weather Factors on the Population Fluctuations of Tuta absoluta (Lepidoptera: Gelechiidae)2312369601010.21608/jppp.2020.96010ENA. M. M.AhmedDepartment of Plant Protection, Faculty of Agriculture, Assiut University (71526), Assiut, EgyptA. A.OthmanDepartment of Physics, Faculty of Science, Assiut University (71526), Assiut, EgyptFrancisco JSolorio SánchezDepartment of Animal Nutrition, Faculty of Veterinary Medicine and animal science, University of Yucatan (UADY), Mérida, Yucatán, México, KM 15.5 carretera Mérida, Xmatkuil Apdo. P. O. BOX 4-116 Itzimna Merida.Journal Article20200615The experiment conducted during (2016/ 2017 and 2017/ 2018) at Fac. of Agric. Farm, Assiut Univ. on tomato hybrids to survey arthropods, determine reduction of <em>Tuta absoluta</em> by Proclaim<sup>®</sup> 5% (S<sub>0</sub>= 0.3 mm) and nano-formulations (S<sub>1</sub>= 17.43± 8.20nm and S<sub>2</sub>= 9.75± 3.86nm) by (120 gm/ Fadden) after 5<sup>th</sup>, 7<sup>th</sup>, and 10<sup>th</sup> days of post-treatment under some weather factors (WF): [Daily Maximum Temperature (DMax.T), Daily Minimum Temperature (DMin.T), and Daily relative humidity (DRH%)]. Survey presented nine insect species under nine genera, three mites, three predators and one parasitoid. The highest grand means for mines and immature stages in 18<sup>th</sup> of Jan., 2017 and 14<sup>th</sup> of March, 2018, and lowest in 3<sup>rd</sup> of Nov., 2016 and 4<sup>th</sup> of Dec., 2017. Meteorological data in 1<sup>st</sup> season were significant with mines and no significant with larvae on hybrids. In 2<sup>nd</sup> season, mines regulated by DMax.T (0.447) and DRH% (-0.621) for Vera, and DRH% (-0.540) for 010 with no significance between weather factors and larvae on hybrids. S<sub>2</sub> in the 1<sup>st</sup> season highly reduced larvae after 10<sup>th</sup> days (103 ± 18.40 and 69.14 ± 14.21; for Vera and 010), and the lowest by S<sub>0</sub> for Vera (10.40 ± 2.22) after 7<sup>th</sup> days; and 22.40 ± 1.78 for 010 in 5<sup>th</sup> days. In 2<sup>nd</sup> season, highest reduction by S<sub>2</sub> (78.64 ± 1.27 and 105.00 ± 6.34 for Vera and 010 in 10<sup>th</sup> days; and lowest by S<sub>2</sub> in Vera after 7<sup>th</sup> days (17.00 ± 0.61); and S<sub>1</sub> for 010 after 5<sup>th</sup> days (29.64 ± 0.81).https://jppp.journals.ekb.eg/article_96010_2506a39a01adad2ac690c773d16b160e.pdfMansoura University, Faculty of AgricultureJournal of Plant Protection and Pathology2090-367711420200401Insecticidal Activity of Six Botanical Powders against the Cowpea Seed Beetle Callosobruchus maculatus F. (Coleoptera: Bruchidae)2372409601110.21608/jppp.2020.96011ENH. A.FouadPlant protection Department, Faculty of Agriculture, Sohag UniversityHasnaa B.AbdelmegedPlant protection Department, Faculty of Agriculture, Sohag UniversityA. M. A.SalmanPlant protection Department, Faculty of Agriculture, Sohag University0000-0002-0780-1017Journal Article20200615Six plant powders including <em>Cinnamomum zeylanicum</em> (Lauraceae), <em>Cuminum cyminum</em> (Apiaceae), <em>Curcuma longa</em> (Zingiberaceae), <em>Hyphaene thebaica</em> (Arecaceae), <em>Lawsonia inermis</em> (Lythraceae) and <em>Zingiber officinale</em> (Zingiberaceae) were tested against <em>Callosobruchus maculatus</em> adults at four treatment levels of 20, 40, 60 and 80 gm of plant powders per Kg of cowpea seeds. Lethal concentration was determined at 24, 48 and 72 h after treatment, and lethal time was determined at 80 gm/Kg<sup>-1</sup> concentration. The results showed that, all botanical powders had effect on <em>C. maculates</em> and <em>L. inermis</em> was the most toxic, and the least toxic powder was <em>C. zeylanicum</em>. Furthermore, plant powder <em>L. inermis</em> at 80 gm/Kg<sup>-1</sup> achieved a lowest LT<sub>50</sub> value. The tested botanical powders represent valuable tools with potential of integration into the management of <em>C. maculates</em> adults.https://jppp.journals.ekb.eg/article_96011_d24d8ec82fa520d97a86838e3708ad50.pdf