Mansoura University, Faculty of AgricultureJournal of Plant Protection and Pathology2090-367712320210301Effect of Introducing Larval Queen Cell Numbers and Rearing Season on Certain Developmental Features and Wet Weight of Commercially Produced Queens under Damietta Conditions (North Egypt)17718315327710.21608/jppp.2021.153277ENM. A. Abd Al-FattahDept. of Economic Entomology & Pesticides, Fac. Agric., Cairo Univ., Giza, EgyptH. A. El-KadyDept. of Economic Entomology, Fac. Agric., Damietta Univ., Damietta, EgyptNoura Abd Al-HadyDept. of Economic Entomology, Fac. Agric., Damietta Univ., Damietta, EgyptJournal Article20210303The present studies were carried out to investigate the quantity (as % of accepted larvae, sealed queen cells and emerged queens) and quality (as weight of queen at emergence) of queen honeybees as affected by some internal environmental factors related to rearing process during late winter period and summer season of two successive years, (2018/2019) at Damietta governorate, (North Egypt). No significant difference was found for all parameters between the two studied years. Significant increase in the percentages of acceptance, sealed queen cells and emerged queens as well as weight of queens at emergence were obtained when introducing 32 than 48 larval queen cells into starter and finisher queen rearing colony, respectively. Larval queen cells which presented on the middle position, (174.6 mg./queen) of rearing bar produced more frequently queens with heavy weight than those presented on the peripheral ones, (169.9mg./queen). The artificially reared virgin queens during summer season were significantly heavier (175.7 mg. /queen) than those reared during the period of late winter, (166.9 mg./queen).Mansoura University, Faculty of AgricultureJournal of Plant Protection and Pathology2090-367712320210301Effect of Genotype of Grafted Larvae and Rearing Bar Level on some Economic Traits in Commercial Production of Honeybee Queens under Damietta Governorate Conditions, North Egypt18519015328010.21608/jppp.2021.153280ENH. A. El-Kady,Dept. of Economic Entomology, Fac. Agric., Damietta Univ., Damietta, EgyptM. A. Abd Al-FattahDept. of Economic Entomology & Pesticides, Fac. Agric., Cairo Univ., Giza, EgyptNoura Abd Al-HadyDept. of Economic Entomology, Fac. Agric., Damietta Univ., Damietta, EgyptJournal Article20210303The present study was conducted in the privet apiary established at Kafr Saad distances, Damietta Governorate, North Egypt, during summer season of 2019, (July and August). The experiments were carried out to determined the effect of genetic origin of the grafted larvae, (<em>Apis mellifera carnica</em> and <em>Apis mellifera bukfast</em>) and level of bar at which the queen cells hung within rearing frame, (top, medium and bottom levels) and the position of these cells on the rearing bar (middle and peripheral positions) onsome output parameters concerned in commercial queens production. The genetic origin of larvae used in grafting process has a positive and significant effect on their acceptance percentage and the live weight of the produced queens when nursed by related starter and finisher building colonies. The percentages of acceptance, sealing and queen emergence as well as the weight of newly emerged virgin queens slightly influenced by the level and position at which the cells presented. In general, queen cells on the bottom bar and middle position were attained a little performance than the others in the commercial queen production during summer season of North Egypt.Mansoura University, Faculty of AgricultureJournal of Plant Protection and Pathology2090-367712320210301Why the Mulberry Silkworms (Bombyx mori) are Monophagous and the Cotton Leaf Worms (Spodoptera littorallis) are Polyphagous?19119516642310.21608/jppp.2021.64060.1015ENSawsan MohamedAbdelmegeedDepartment of plant protection, Faculty of Agric., Ain Shams University, Cairo, EgyptJournal Article20210325Larvae of order Lepidoptera have biting mouth parts and almost fed on leaves. The insects of Lepidopterous were divided into three groups depended on number of the host plants, the monophagous, the oligophagous and the polyphagous. Sense organs are responsible for the number of host plants and insects select for hosts to fed on. In this study, the head capsule of larvae was cut at the end of larval stage of the mulberry silkworms and the cotton leaf worms to examine by scanning electron microscope for the number and type of sense organs on head capsule of larvae of both insects. The total number of sense organs on the head capsule of the mulberry silkworm was 82 where they were more than that of the cotton leaf worm where they were only38 sense organs. In addition, When mouth parts of the mulberry silk worm were covered with nail polish then the larvae were offered five different species of plant leaves ,<em>Abelmoschus esculentus</em> (okra), <em>Vitis vinifera</em> (grapes), <em>Ricinus communis</em> (castor bean plant),<em>Cucurbita moschata</em> (Pumpkin) and <em>Morus alba</em> (mulberry) to fed on. The treated larvae couldn’t recognize mulberry leaves to feed on. The larvae fed on small part of grapes and okra leaves then stopped to continue feeding. Vomiting and diarrhea were happened to larvae then died.Mansoura University, Faculty of AgricultureJournal of Plant Protection and Pathology2090-367712320210301Population and Mortality Studies on the Black Scale Insect, Parlatoria ziziphi (Lucas) (Hemiptera: Diaspididae) in an Egyptian Citrus Orchard19720215327310.21608/jppp.2021.153273ENS. S.AwadallaEconomic Entomology Department, Faculty of Agriculture, Mansoura University, EgyptM. H. BayoumyEconomic Entomology Department, Faculty of Agriculture, Mansoura University, EgyptM. El-MetwallyPlant Protection Research Institute, Agricultural Research Center, Giza, EgyptRania M.AlhussienyPlant Protection Research Institute, Agricultural Research Center, Giza, EgyptJournal Article20210303The diaspidid black scale, <em>Parlatoria ziziphi</em> (Lucas) is amongst the most destructive pests to citrus in several countries. This study was conducted in navel orange orchard at the experimental farm belonging to Mansoura University during 2017/2018 and 2018/2019. <em>Parlatoria ziziphi</em> had three peaks of abundance annually. The highest peaks occurred in autumn. The ectoparasitoid, <em>Aphytis </em>sp < em>. had four peaks of abundance annually and its populations were synchronized with those of the insect. The endoparasitoid, <em>H. aspidioti</em> exhibited three-four peaks of abundance annually and the parasitoid populations were coincided partially with those of the insect. However, as the population of insect increased, the parasitism rates by <em>Aphytis</em> was significantly decreased, whereas those of <em>H. aspidioti</em> were unstable. Thus, <em>Aphytis</em> seems to be inverse-density mortality agent and its releases must be early in the season on low pest populations. Parasitism% by <em>Aphytis </em>sp. had two peaks annually, almost occurred in the period from May to August. <em>Aphytis</em> parasitism percentages ranged from 14.6 to 35.4%, whereas those of <em>H. aspidioti</em> ranged from 5.7 to 16.5% in both years. <em>Harbolepis aspidioti</em> recorded three peaks of parasitism in January, May, and August annually with the highest one was in January. Predation% in <em>P. ziziphi</em> populations ranged from 5.8 to 18.9%. The highest percentages of unknow mortality (12.3-17.8%) were in the period from December to January annually. The total population mortality ranged from 37.9 to 73.9%. Both parasitoid species contributed with the highest fate in the total population mortality of <em>P. ziziphi</em> in all seasons.Mansoura University, Faculty of AgricultureJournal of Plant Protection and Pathology2090-367712320210301Effectiveness Buminal-Ammonia Mixtures on Attraction of the Mediterranean Fruit Fly, Ceratitis capitata with Particular Emphasis on their Hidrogenionic Potential (pH)20320815354610.21608/jppp.2021.153546ENMohamed A. K.HassanFaculty of Agriculture - Mansoura University.29801031603384S. S. AwadallaEconomic Entomology Department, Faculty of Agriculture, Mansoura University, EgyptM. M. El-MetwallyPlant Protection Research Institute, Agricultural Research Center, Giza, EgyptRania M.AlhussienyPlant Protection Research Institute, Agricultural Research Center, Giza, EgyptJournal Article20210304Food bait which is rich in nitrogen has an intensive impact on physiology and behavior of fruit flies. Addition of ammonia to food baits can improve their effectiveness and the pH-level of these baits plays an important role in attracting fruit flies, since the effectiveness of bait is diminished by decreasing the pH-level. Therefore, the current study examined the effectiveness of several mixtures of the protein-based bait, Buminal, and ammonia compounds (ammonium acetate, ammonium chloride, di-ammonium phosphate and phosphoric acid) in attracting the Mediterranean fruit fly, <em>Ceratitis capitata</em>. Traps provided with each of these different mixtures were hanged in two fruit orchards (Novel orang and Mandarin) and examined in three-day intervals for 12 day period. In each inspection time, fifty milliliters of each mixture in the field were taken to estimate the pH-level. Regardless, the concentration of ammonia compounds, addition of di-ammonium phosphate to Buminal attracted more fruit flies than other mixtures. Further, all the tested mixtures attracted more females than males in both orchards. Although, the highest captures of <em>C. capitata</em> were by mixture of Buminal 5%+di-ammonium phosphate 1%, it did not coincide with changing in pH levels of this mixture. Only, the changes in pH-level of Buminal 5%+di-ammonium phosphate 2% led to significant increase in the number of trapped flies. This study might suggest that increasing the pH-level of food lure above 7.6 might adversely decline the number of trapped flies. Buminal+di-ammonium phosphate mixture can be generalized in IPM programs for <em>C. capitata</em> in Egyptian fruit farms.Mansoura University, Faculty of AgricultureJournal of Plant Protection and Pathology2090-367712320210301Comparative Efficacy of Different Chemical Fungicide Groups against Cotton Root Rot20921415357110.21608/jppp.2021.153571ENFathia SolimanAlshorakyplant pathology instetute,agriculture research centerJournal Article20210304The current study was undertaken to evaluate the influence of various fungicidal treatment on the inhibition percent of pathogens and as seed treatment on cotton seedling stand establishment against root rot of cotton in <em>in vitro</em> as well as in field conditions at Sakha Agriculture Research Station during two seasons 2019 and 2020. This study was carried out to evaluate once more available fungicides, for their effectiveness against the root rot pathogens (<em>Rhizoctonia solani ,Fusarium oxysporum </em>and<em> Macrophomina phaseolina</em>). And upon it evaluate which of them for used it as seed treatment. All tested fungicides exhibited reduction in the radial growth of mycelium for all the testing fungi significantly compare with control. Hymexazole and Tebuconazole + fludioxnile showed excessive decreased of mycelial growth, when Hymexazole; Tebuconazole + fludioxnile; Carboxin + thiram; Triticonazole and Fludioxanile + mefenoxam (metalaxyl-m) gave best results in the reduction percent of inhibition) for the three tested pathogens. Efficiency of fungicide seed treatments were expressed as increased in standing percent; plant height and dry weight of plant. Disease incidence was very low in both Propanocarb HCl + fostyl Al and Fludioxanile + mefenoxam (metalaxyl-m). Generally, the fungicide treatments ended a little or no difference in plant height or dry weight. Under the field conditions (low disease pressure), results indicated that, fungicide seed treatments are slightly effective in increasing standing percent. Diniconazole-M was the most effective in disease reduction (expressed as 100% standing percent). These treatments were translated into increased plant height and dry weight of plants.Mansoura University, Faculty of AgricultureJournal of Plant Protection and Pathology2090-367712320210301Side Effect of Indigenous Entomopathogenic Fungi on the Predatory Mite, Cydnoseius negevi (Swirski and Amitai) (Acari: Phytoseiidae)21522315548010.21608/jppp.2021.66659.1020ENNouran Mohamed SaadSaadzoology,agriculture,mansoura.universityA. A. YousefDept. Agric. Zool., Fac. Agric. Mansoura Univ., EgyptA. H. FoulyDept. Agric. Zool., Fac. Agric. Mansoura Univ., EgyptJournal Article20210310The present study deals with the side effect of the four entomopathogenic fungi EPF, <em>Metarhizium anisoplia</em> (Metchnikoff), <em>Beauveria bassiana</em> (Balsamo), <em>Paceliomyces fumosoroseus</em>(Vassiljevsky) and <em>Cladosporium cladosporiodes</em> (Fresen), which used mainly against insect pests and spider mites, on the phytoseiid predatory mite, <em>Cydnoseius negevi</em>(Swirski and Amitai) in laboratory. Spore suspensions of LC<sub>50 </sub>(1x10<sup>6</sup>=10<sup>6</sup> conidia/ml) of the tested fungi, which achieved a significant reduction% in populations of the two-spotted spider mite, <em>Tetranychus urticae</em> Koch, were sprayed on different stages of <em>C. negevi </em>individuals. In general, a slight negative toxic effect of EPF observed where no adverse effect was noticed on eggs while a negative effect was increasingly observed on larvae, protonymps and deutonymphs. Mortality percentage of treated adult females increased by increasing time after treatment, where the highest mortality achieved 8 days after exposure. <em>Paceliomyces fumosoroseus</em> had the highest rate of reduction in egg production and followed by <em>B. bassiana</em> then <em>M. anisoplia</em>, while <em>C. cladospriodes</em> came last. Same ranking was observed regarding the life table parameters, where LC<sub>50</sub> of <em>P. fumosoroseus</em> caused the lowest R<sub>o</sub>, r<sub>m</sub> and e<sup>rm</sup>values and prolonged the mean generation time T and doubling timeD<sub>t </sub>(days). Accordingly, in IPM program against insect and mite pests, we should use EPF as biocontrol agents, that are safe to biocontrol agents.Mansoura University, Faculty of AgricultureJournal of Plant Protection and Pathology2090-367712320210301First Detections of Benzoquinone in Stored Wheat Grains Infested by Sitophilus granarius L. (Coleoptera: Curculionidae) during Storage22522815548110.21608/jppp.2021.155481ENH. B. H. HussainPlant Protection Research Institute, ARC, Dokki- Giza, EgyptJournal Article20210310The current study was carried out to investigate the effect of stored grains infestation with<em> S</em>.<em> granarius</em> and storage periods on detection of toxicants secretion as benzoquinones. The results showed that loss of wheat grains significantly increased with increasing storage periods and different insect densities. The highest losses percentagecaused by<em>S. granarius</em> was 6.95 % at densities of 20 pairs. The results showed there is only Ethyl 1,4-benzoquinone was the toxic compound detected in all insect densities and storage periods. The benzoquinones secreted by <em>S. granarius</em> adults increased with an increase of the <em>S. granarius</em> density. The highest values were recorded 313.9 and 394.78 ± 9.33 µg/g wheat flour with the highest insect density (20 pairs) at 2 and 4 storage periods resp. Also reported results, the concentration values of ethyl 1,4 benzoquinone per insect were 0.55 ± 0.04 and 0.35±0.03 µg/g wheat flour with the highest insect density (20 p) at 2 and 4 storage, resp. We can confirm that the organic compound ethyl 1,4 benzoquinone was coming from <em>S. granarius</em> as defensive secretions.Mansoura University, Faculty of AgricultureJournal of Plant Protection and Pathology2090-367712320210301Diadegma aegyptiator Shaumer 1966 (Hymenoptera: Ichneumonidae): New Record Parasitoid on the Rib Miner, Scrobipalpa ocellatella Boyd. (Lepidoptera: Gelechiidae) in Egyptian Sugar Beet Fields22923115579010.21608/jppp.2021.155790ENK. G. BazazoPlant Protection Research Department, Sugar Crops Research Institute, Agricultural Research Center, Egypt.H. M. HassanDepartment of Economic Entomolgy, Faculty of Agriculture, Kafr El-Sheikh University, 33516, Kafr El-Sheikh, Egypt.Journal Article20210311The larvae of the rib miner<em>,Scrobipalpa ocellatella</em> <em>Boyd </em>feed on the sugar beet leaves and roots causing huge damage in such a way that the root rot and thus it caused a reduction in the yield of roots and the sugar content in the roots. Parasitoids are the most effective natural enemies for insect biological control. In nature, several parasitoids were observed to be potential bio-agents of serious crop pests. The future of "insecticide-less" pest management will be driven by a bouquet of parasitoids and predators complimented by entomopathogens. The reduction in insecticide residues in food - sugar beet is a food crop - is desirable for public health. The present study was carried out at the experimental farm of Sakha Agricultural Research Station, Kafr El-Sheikh Governorate-Egypt throughout two successive seasons 2018/2019 and 2019/2020. New parasitoid was recorded from the rib miner pupae. It was identified as <em>Diadegma aegyptiator </em>on <em>S.ocellatella </em>for the first time in Egypt. The Overall of parasitism were 24.52 and 31.03% in the two seasons, respectively. As well as, statistical analysis proved that a highly positive significant correlation coefficient values between <em>S.ocellatella </em>and its parasitoid in two seasons, respectively.These results elucidate that this parasitoid is a promising tool in reducing <em>S.ocellatella </em>populations.Mansoura University, Faculty of AgricultureJournal of Plant Protection and Pathology2090-367712320210301Studies on Certain Piercing-Sucking Insects Infesting Potato Plants and their Associated Predators in Dakhlia Governorate., Egypt23323815739510.21608/jppp.2021.157395ENA. A.GhanimEconomic Entomology Department, Faculty of Agriculture, Mansoura University, Egypt.Hala A.El-SerafyEcon. Entomol. Dept., Fac. of Agric., Mansoura Univ., EgyptHoria A.HassanPlant Prot. Res. Institute, Agric. Res. Center, DokkiNadia E.MohamedPlant Prot. Res. Institute, Agric. Res. Center, DokkiFaten B.Abu El MattiPlant Prot. Res. Institute, Agric. Res. Center, DokkiJournal Article20210317Field trials were conducted to study the dominant piercing-sucking insect species infesting potato plants in Dakahlia Governorate. The obtained results indicated that there were six piercing-sucking insects attacking potato plants and the dominant piercing-sucking insects were <em>Myzus persicae</em> (Sulker); <em>Aphis gossypii</em> Glover; <em>Empouasca descipiens</em> Polai. and <em>Bemisia tabaci</em> (Gennadius) during the two years of study. Data cleared that <em>M. persicae</em> and <em>A. gossypii</em> had one peak in each potato plantation and <em>E. descipiens</em> recorded two peaks in summer plantation, in the meanwhile three peaks in winter plantation during the two years of investigation. <em>B. tabaci</em> had three peaks in summer and winter plantation during the period of study. The obtained results revealed that there were four predators belonging to Family Coccinellidae namely: <em>Hippodamia trideciapunctata</em> L.<em>, Coccinella undecimpunctata</em> L.<em>, Chilomenes propinqua isis</em> (Mulsant) and <em>Chilomenes propinqua nilotica</em>(Mulsant), and one predator from family Chrysopidae, namely <em>Chrysoperla carnea</em> (Steph.) was recorded. It can be stated that most dominant species were <em>H. tridecimpunctata</em> and<em> C. undecimpunctata</em>. <strong> </strong>Mansoura University, Faculty of AgricultureJournal of Plant Protection and Pathology2090-367712320210301Comparative Effect of Three Entomopathogenic Fungi against Whitefly Bemisia tabaci (Gennadius) Infesting Eggplant under Field Conditions at Kafr El-Sheik Gov., Egypt23924415740410.21608/jppp.2021.157404ENMaha S.NadaPlant Protection Research Institute, Agricultural Research Center, Cairo, EgyptHala A.Central Laboratory for Organic Agriculture, ARC, EgyptS. A.GaffarCentral Laboratory for Organic Agriculture, ARC, EgyptA. TamanPlant Protection Research Institute, Agricultural Research Center, Cairo, EgyptJournal Article20210317Using entomopathogenic fungi, as a powerful bio-control agent, is considered one of the promising methodologies in IPM programs. The current investigation aimed to test the efficiency of <em>Metarhizium anisopliae, Verticillium lecanii, and Beauveria bassiana</em> against whitefly on Eggplant (variety Anann) in Kafr El Sheikh Governorate, Egypt. The experiment was laid in a randomized complete block design, in an area of one feddan, divided into 4 plots (5 replicates each). The study extended from July till September, during two successive seasons (2019 and 2020) and each fungal product was sprayed four times (one spray/week). Temperature fluctuated from 29 to 35 ̊C and RH ranged from 49 to 52%. Results indicated that using entomopathogenic fungi significantly reduced <em>B. tabaci</em> population. <em>V. lecanii</em> was the most influential fungus, as it caused the highest control percentage compared to the other two fungi and control treatment. Repeating application during 4 successive weeks raised fungi effectiveness and caused ascending mortality levels in <em>B. tabaci </em>population. Production rates were in line with percentage reductions of whiteflies. The largest yield of eggplant’s fruits was produced in the plots treated with <em>V. lecanii</em>. Accordingly, the study recommends using <em>V. lecanii</em> in whiteflies control strategies in Egypt. Moreover, further investigations are needed to study compatibility of <em>V. lecanii</em> with other bio-agents to avoid inconsistency when applied together in pest management programs.Mansoura University, Faculty of AgricultureJournal of Plant Protection and Pathology2090-367712320210301The Relative Potency of some Pesticides against Cotton Leafworm, Spodoptera littoralis (Boisd.) and their Side Effect on the Natural Enemy, Chrysoperla carnea (Stephens)24525015739910.21608/jppp.2021.157399ENReda RAbdullahplant protection research institute, Agriculture research center, Dokki, GizaJournal Article20210317The cotton leafworm, <em>Spodoptera littoralis</em> (Boisd.) is one of the dangerous economic insects on different crops in Egypt because it causes more damages in crops especially cotton and leafy vegetables. On the other hand, the natural enemy, <em>Chrysoperla carnea</em> (Stephens) is an important predator in all fields because it is able to control many insect pests and mites. In this study, six commercial pesticides (Chlorpyrifos, Alpha-cypermethrin, Methomyl, Abamectin, Biossiana, and Dipel) and two new compounds under the study (Crude extract of <em>Beauveria bassiana</em> metabolites and synthesis compound from Cyanoacetamide derivatives) were tested to determine their toxicity against the 2<sup>nd</sup> instar larvae of <em>S. littoralis</em> and their side effects against the 2<sup>nd</sup> instar larvae of <em>C. carnea. </em>All tested compounds had different degrees of toxicity against both tested insects. Based on the values of LC<sub>50 </sub>, toxicity index and relative potency, Alpha-cypermethrin was the highest toxic pesticide against <em>S. littoralis</em> and <em>C. carnea</em> followed by Methomyl and Chlorpyrifos but Biossiana was the least toxic one. In addition, the toxicity of tested chemical pesticides against <em>C. carnea</em> was more than their toxicity against <em>S. littoralis</em>. While, the toxicity of tested biopesticides against <em>C. carnea</em> was less than their toxicity against <em>S. littoralis</em>. In general, the sensitivity of the predator <em>C. carnea</em> to the tested biopesticides was less in comparison with chemical pesticides. Therefore, this study recommends using biopesticides besides the slight using of chemical pesticides to preserve the natural enemies and environment and achieve the best pest control.<strong> </strong>Mansoura University, Faculty of AgricultureJournal of Plant Protection and Pathology2090-367712320210301Bioactivity of Moringa oleifera and Ruta angustifolia Oils on Spodoptera littoralis (Boisd.) Moths’ Vitality25125716069010.21608/jppp.2021.160690ENRehab M.El-GendyPlant Protection Research Institute, Agriculture Research Center (ARC), Dokki, Giza, Egypt.Hend M.SabryPlant Protection Research Institute, Agriculture Research Center (ARC), Dokki, Giza, Egypt.Journal Article20210303<em>Spodoptera littoralis</em>, cotton leafworm is a destructive pest for several economic crops. The more use of chemical pesticide the more increase of this pest resistance, that lead to discovering new natural pesticides from plant extracts and oils. The present search is targeted to suggest alternative components from natural sources that more safety to the ecosystem and had the ability to reduce insect prohibition. The experimental results exhibited highly significance effect on adult moths’ vitality aspects with the pre-pupal treatments by <em>Moringa oleifera and Ruta angustifolia</em>at all tested concentration especially 4% concentration as shorten female moths' longevity and reduced the oviposition period. Obviously, moringa recorded the shortest oviposition period (3.00 days) while ruta oil recorded (5.33 days) compared to control (10.66 days). Significantly reduced fecundity and hatchability and increased the sterility% compared to control. The tested plant oils caused severe ovarian and testicular histological deformation and significant increase in total lipids and total carbohydrate contents also, marked DNA fragmentation in variable levels in ovaries and testes in each oil treatments at 4% concentration. The promising results of both oils on <em>S. littoralis</em> reproductive system can prove them be used in the integrated management programs of this pest.Mansoura University, Faculty of AgricultureJournal of Plant Protection and Pathology2090-367712320210301Effect of Different Control Agents on Meloidogyne Incognita Kofoid Infesting Cucumber Plants25926816069410.21608/jppp.2021.66215.1019ENR. M. El-AshryPlant Prot. Dept., Fac. of Agric., Zagazig Univ., Zagazig, EgyptM. A. I.YoussifPlant Protection Research Institute , Dokki, EgyptA. E. A. M. ElsobkiPlant Prot. Dept., Fac. of Agric., Zagazig Univ., Zagazig, EgyptSherin M. M. Y.HelalyPlant Prot. Dept., Fac. of Agric., Zagazig Univ., Zagazig, EgyptJournal Article20210303The stock solution 10% and half stock solution 5% of three oils namely; mineral oil (Diver oil 97 % EC), plant oils marjoram oil (<em>Origanum majorana </em>L.) and orange oil (<em>Citrus aurantium</em> L.), as well as two species of bacteria <em>Bacillus megaterium</em> and BTS<sub>1</sub> (<em>Bacillus poylmyxa</em>)at two concentrations showed that nematicidal activity against egg-mass and second stage juveniles of <em>Meloidogyne incognita</em>Kofoid <em>in vitro</em> experiments. Egg hatching and juvenile mortality were significantly (<em>P </em>≤ 0.05) influenced by tested materials, concentration and exposure time. <em>B. megaterium</em>, diver oil and marjoram oil gave a higher effect, while <em>B. poylmyxa </em>was the lowest effective one. In case of juvenile mortality, <em>B. poylmyxa</em>, diver oil and marjoram oil gave the highest percentage of juvenile mortality (75.14, 73.00 and 61.85 %) after exposure at stock solutions (10%) and (53.00 , 67.42 and 52.57 %) with exposure to half stock solution (5%) after seven days of treatment, respectively. Under greenhouse conditions<strong>,</strong> results indicated that, oxamyl, dry leaf powder of moringa and <em>B. megaterium</em> were the most effective in suppressing root galling of <em>M. incognita</em> infectingcucumber plants. The maximum percentage of increase in shoot fresh weight was recorded on cucumber 43.26 and 25.36% in pots treated with oxamyl and <em>B. megaterium</em>, respectively. A moderate effect on treated eggs with the tested eco-friendly materials was observed on invasion and reproduction of <em>M.incognita</em> under greenhouse conditions. All tested materials significantly (<em>P </em>≤ 0.05) reduced nematode parameters and increased cucumber plant growth parameters as compared with control treatment. Strongly decrease in number of egg masses and galls diameter was recorded in pots treated with oxamyl, moringa and <em>B. megaterium</em> followed by oils. Minimum number and gall diameter particularly ≥ 4mm was recorded in cucumber plants treated by oxamyl, moringa and <em>B. megaterium</em>. Data showed that moringa and <em>B. megaterium</em> could be used to increase crop yield of cucumber plants and for controlling root – knot nematode, <em>M. incognita</em>.