Mansoura University, Faculty of AgricultureJournal of Plant Protection and Pathology2090-36779920180901Isolation, Identification and Biological Activities of Endophytic Fungi from Egyptian Medicinal and Aromatic plants5435464375510.21608/jppp.2018.43755ENMona M. G.SaadDepartment of Pesticide Chemistry and Technology, Faculty of Agriculture, 21545-El-Shatby, Alexandria University, Alexandria, EgyptKessma M.AliDepartment of Pesticide Chemistry and Technology, Faculty of Agriculture, 21545-El-Shatby, Alexandria University, Alexandria, EgyptA. E.KornaDepartment of Pesticide Chemistry and Technology, Faculty of Agriculture, 21545-El-Shatby, Alexandria University, Alexandria, EgyptJournal Article20190804Eight endophytic fungi were isolated from three Egyptian plants (<em>Chenopodium album, Pelargonium graveolens</em> and<em> Malva parviflora</em>). The fungi endophytes were identified by morphological and molecular methods as<em> Fusarium chlamydosporum</em> MG786540,<em> Fusarium oxysporum</em> MG786541, <em>Alternaria alternata </em>MG786542, <em>Alternaria solani</em> MG786543<em>, Fusarium equiseti</em> MG786544<em>, Alternaria alternata </em>MG786545, <em>Stemphylium sp </em>and <em>Phoms </em>sp. The endophytic fungi isolates exhibited antagonistic activities to phytopathogenic fungi by dual culture bioassay. <em>A. alternata</em> (MG786545) caused 32.58% and 44.68% growth inhibition of <em>Fusarium oxysporum</em> and <em>Phytophthora infestance</em>, respectively. <em>F. equiseti</em> (MG786544) caused 34.64% growth inhibition of <em>Alternaria solani</em>. Spore suspension of <em>A. solani</em> (MG786543), <em>A. alternata</em> (MG786545) and<em> Stemphylium </em>sp endophytes were tested for their herbicidal activity and plant growth promotion.<em> A. solani</em> (MG786543) at10<sup>8</sup> spore/ ml inhibited 33.1% of seed germination and reduced 31.3%, 23.1% of shoot and root growth of <em>Lolium temulentum</em>, respectively. <em>Stemphylium sp</em> at10<sup>8</sup> spore/ ml had the ability to enhance <em>Triticum aestivum</em> growth by increasing shoot and root lengths, and fresh and dry weights comparing to control.Mansoura University, Faculty of AgricultureJournal of Plant Protection and Pathology2090-36779920180901Population Fluctuation of the Peach Fruit Fly, Bactrocera zonata (Saunders) and the Mediterranean Fruit Fly Ceratitis capitata (Wiedemann) (Diptera, Tephritidae) on three Grape Varieties in Nubaria , Egypt.5475554375710.21608/jppp.2018.43757ENSeham M.ElmahdyPlant Protection Institute, A.R.C., Dokki , Giza, 12618 EgyptY. E.AfiaPlant Protection Institute, A.R.C., Dokki , Giza, 12618 EgyptJournal Article20190804The population fluctuation of the peach fruit fly (PFF), <em>Bactrocera zonata </em>and the Mediterranean fruit fly (MFF), <em>Ceratitis capitata </em>ondifferent grape varieties (White grape variety (Sbaror), Red grape variety (Filam) and Black grape variety (Autumn royal) were studied throughout three successive seasons 2014-2016, starting from March to September and represented by catch/trap/ daily of males on three different varieties at Nubaria district. Results revealed that the population density was the most abundant during the third season in the Sbaror, Filam and autumn royal varieties. During the three seasons, the population density of MFF was more than of PFF in all varieties.<em> Each of B. zonata </em>and<em> C</em><em>.</em><em> capitata</em>have one peak on Sbaror and Filamand Autumn royal varieties through period from May to July.Seasonal activity of fruit fliesrepresented by total monthly catch of males on three varieties of grape during three successive seasons recorded the highest peak of seasonal abundance on May during the third season with total monthly catch 1050.8 males/month representing 59.2% , while the total monthly catch of males during the two seasons were more in June were 160.46 and367.3 males/month representing 42.64 and 55% from total population, respectively. Results obtained of seasonal fluctuation of <em>B. zonata</em> and <em>C.capitata</em> on three varieties grapesrepresented by total numbers and percentages between varieties were more on Sbaror and Filam than autumn royal where the total population for variety through three seasons 2050.38, 547.84 and 214.79males,were recorded, respectively. The results showed that the percentage of <em>C</em><em>.</em><em> capitata</em>was high in the third season than second and first seasons, on the other hand the percentage of <em>B. zonata</em> was more in the first season than second and third season in all varieties. The highest total numbers of males on' Sbaror variety was 1335.9, 467.8 and 249.67 males /seasons representing 98.73, 87.96 and 79.80% for C.C and 1.27,12.04 and 20.20% for <em>B. zonata</em> of total captured males, during the third, second and first seasons, respectively, while the lowest total number on Autumn royal variety was (96.4,69.4&48.99 males/season) representing (92.12,75.65& 68.09%) and (7.88, 24.35&31.91 %) for <em>C.C and B.Z</em> during third, second and first seasons, respectively. Statistical analysis of variance in 2014 -2016 season showed that combined effect of the weather factors to CTD revealed EV were positively significant, in Sbaror variety, the simple correlation between maximum and minimum temperatures and CTD number were high significant and significant and the percentage ofexplained variance (E.V %) were high significant and significant were 46.02 and 26.78% for during first year and second year for <em>B. zonata</em>.<em> </em>While the simple correlation between and relative humanity and CTD were positive significant for<em> C. capitata </em>during the third years.<strong> </strong>During 2015, the simple correlation between maximum temperatures and CTD number in Filam variety was significant during second year and the percentage of explained variance (E.V.%) were significant its 26.77 and 28.14% for <em>B. zonata</em> and <em>C. capitata</em>, respectively. In Autumn royal variety, the simple correlation between maximum and minimum temperatures and CTD number were significant and high significant during first season and the percentage of explained variance (E.V.%) were 26.75% for <em> B. zonata</em>, respectively<strong>. </strong> Mansoura University, Faculty of AgricultureJournal of Plant Protection and Pathology2090-36779920180901Monitoring of Tricyclazole and Isoprothiolane Residues and their Effects on Blast Disease, Yield and its Components, Grain Quality and Chemical Components of Rice5575664376010.21608/jppp.2018.43760ENRabab M.ElamawiRice Pathology Department, Plant Pathology Research Institute, Agricultural Research Center, Sakha, Kafrelsheikh, 33717, EgyptFatma A.MostafaIntegrated Pest Management Department, Plant Pathology Research Institute, Agricultural Research Center, Giza, EgyptR. A. S.El-ShafeyRice Research and Training Center, Field Crop Research Institute, Agricultural Research Center, Sakha, Kafrelsheikh, 33717, EgyptJournal Article20190804The systemic fungicides tricyclazole (Beam 75% WP) and isoprothiolane (Fuji-one 40% EC) are widely used to control<em> Pyricularia</em><em> oryzae</em> infection causing rice blast disease in Egypt. A field experiment was conducted at Rice Research and Training Center farm, Sakha, Kafrelsheikh, Egypt during 2015 and 2016 seasons on susceptible cultivar Sakha 101. The impact of tricyclazole (0.5 mg/L) and isoprothiolane (2 ml/L) on <em>P. oryzae</em> infection, yield and its components, grain quality and chemical components of rice were evaluated. Meanwhile, the fungicides residue dynamics on rice straw, husk and milled rice were determined using Quick Easy Cheap Effective Rugged and Safe (QuEChERS) method combined with HPLC-MS/MS detection after 30 and 60 days post-harvest. The results indicated that tricyclazole and isoprothiolane were significantly possessed a high level of controlling blast disease whereas, both fungicides reduced disease severity, areas under disease progress curve (AUDPC), and rate of leaf blast progress (r-value) disease parameters in both seasons. Residue analysis of tricyclazole in rice straw (1.06 mg/kg) was higher than the residue in the husk and milled rice (0.19 and 0.14 mg/kg, respectively) after 30 days post-harvest. The residues of tricyclazole in the husk and milled rice were lower than the Maximum Residue Limit (MRL). Also, isoprothiolane residue analysis in milled rice was 0.58 mg/kg after 60 days post-harvest and below the MRL. Also, most of the yield and yield components were significantly increased by the application of both fungicides over the untreated plots. Tricyclazole and isoprothiolane treatments enhanced the grain chemical components concentration as carbohydrates, fats, ash, and fiber. Finally, the investigation revealed that tricyclazole and isoprothiolane were effective against rice blast disease and considered safe fungicides according to the European Food Safety Authority. Based on our residue analysis results and Egyptian rice consumption, we suggested that determine our own Egyptian MRL. Whereas, our total rice consumption reaching 4 million ton/year in 2016, it was considered fourth fold than Europe consumption (1 million ton/year).Mansoura University, Faculty of AgricultureJournal of Plant Protection and Pathology2090-36779920180901Antimicrobial Activity of Artemisia herba-alba Extract against Pathogenic Fungi of Pigeon Droppings5675714376710.21608/jppp.2018.43767ENHend S.GhareebPlant Protection Research Institute, Dokki, Giza, EgyptM. A.M.Plant Protection Research Institute, Dokki, Giza, EgyptJournal Article20190804Pigeon droppings represent the most important source of pathogenic fungi of plants and other living organisms. The occurrence of fungi in samples of feral pigeon droppings was screened. Moreover, the antifungal properties of <em>Artemisia</em> <em>herba</em>-<em>alba</em> extract against these fungi was investigated. Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of this extract were determined. The results revealed that <em>Alternaria</em> <em>alternata</em> recorded the highest frequency in the collected samples while, <em>Cladosporium</em> <em>sphaerospermum</em> was the least isolated fungus. <em>Artemisia</em> <em>herba</em>-<em>alba</em> extract showed the highest antifungal effect against <em>C</em>. <em>sphaerospermum</em> fungus by MIC and MFC values of 1.5% and 3%, respectively. However, <em>A</em>. <em>alternata</em>, <em>Aspergillus</em> <em>niger</em>, <em>Fusarium</em> <em>oxysporum</em> and <em>Rhizopus</em> <em>stolonifer</em> were the most resistant fungi to the extract by MIC value (6%). The highest MFC value (10%) was showed against <em>A</em>. <em>niger</em>, <em>Mucor</em> <em>circinelloides</em>, <em>Penicillium</em> <em>citrinum</em> and <em>R</em>. <em>stolonifer</em> fungal isolates.Mansoura University, Faculty of AgricultureJournal of Plant Protection and Pathology2090-36779920180901Combined Effect of Entomopathogenic Fungi, Beauveria bassiana and Certain Insecticides for the Control of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) under Semi-Field Conditions5735764376910.21608/jppp.2018.43769ENM. F.Abdel AzizPlant Protection Research Institute, ARC, Ministry of Agric., Dokki, EgyptA. A.EbrahimPlant Protection Research Institute, ARC, Ministry of Agric., Dokki, EgyptA. R.El-GabalyPlant Protection Research Institute, ARC, Ministry of Agric., Dokki, EgyptJournal Article20190804This study was carried out at Quesna district, Menoufia governorate during 2017 growing season under semi field conditions against 2<sup>nd</sup> and 4<sup>th</sup> instar larva of <em>Spodoptera littoralis</em> (Boisd.) to evaluate the efficiency of combining <em>Beauveria</em> <em>bassiana</em> with certain insecticides (emamectin benzoate, methomyl and <em>Bacillus thuringiensis</em>) in tomato field. In general, data revealed, clearly, that methomyl had the highest efficacy against 2nd larval instar, where, the percentages corrected mortality were 98.28, 62.68 and 27.09% at concentrations RC,1/2RC and 1/4RC respectively. While, when 4<sup>th</sup> larval instar treated by emamectin benzoate, the observed total percentage corrected mortality was the highest value (90.54%) at RC followed by methomyl (85.79%) at RC, whereas, when treated 2<sup>nd</sup> and 4<sup>th</sup> larval instar with fungi had the lowest values of percentage corrected mortality at all concentrations compared with remaining treatments. When treated larvae with mixture of fungi (<em>B. bassiana</em>) and insecticides percentages mortalities of second and fourth larval instar of emamectin benzoate + <em>B. bassiana</em> and methomyl + <em>B. bassiana</em> had the highest values after 24hrs. then decreasing gradually, but the highest values of percentage mortality of second and fourth larval instar when mixtures of <em>Bacillus thuringiensis</em> + <em>B. bassiana</em> were after fifth days at concentrate of insecticide 1/2RC and after third day at concentrate of insecticide 1/4RC. So mixing increases the efficiency of their insecticides.Mansoura University, Faculty of AgricultureJournal of Plant Protection and Pathology2090-36779920180901Control of Avocado (Persea americana Miller) Damping-off and Root Rot Diseases in Egypt5775854388410.21608/jppp.2018.43884ENM. A.RadwanPlant Pathol. Res. Inst., Agric. Res. Center, Giza, EgyptM. S. S.HassanPlant Pathol. Res. Inst., Agric. Res. Center, Giza, EgyptJournal Article20190805<span>Damping-off and root rot are the most troublesome and common fungi diseases attack avocado (<em>Persea americana</em> Miller) which affecting seedling production and causing serious losses in the number of seedlings in nurseries and transplants in new orchards. Nursery and greenhouse experiments on pre- and post-emergence damping-off of avocado were conducted at</span><span> El-Qanater El-Khairia Horticulture Research Station, Agricultural Research Center throughout 2016 and 2017 growing seasons</span><span>. Hass, Eitinger and Fuerte were the most susceptible cultivars to damping-off and root rot diseases, followed by Duke. Whereas, the lowest percentage of pre- and post-emergence damping-off and the highest percentage of survival plants were recorded on Reed, Benkerton and Bekon. Pathogenicity tests on avocado seeds and seedlings under greenhouse conditions showed that <em>F. solani, F. moniliforme, F. semitectum</em> and<em> L. theobromae</em> which isolated from roots of either seedlings in nursery or transplants in new orchards were pathogenic but varied at different degrees in their pathogenicity. <em>Fusarium solani</em> was the most pathogenic fungi causing the highest percentage of pre- and post-emergence root-rot followed by<em> F</em>. <em>moniliforme. </em>On the other hand, the least pathogenic ones were <em>F. semitectum</em> and<em> L</em>.<em> theobromae</em>. To overcome this problem, single treatment of nine compounds <em>i.e</em>. </span><span>Topsin M7</span><span>0</span><span>WP, DithaneM-45 80%WP</span><span>,</span><span> Kema-Z</span><span> 50% WP, Rizolex–T 50% WP,</span><span> Salicylic acid</span><span>, Bio Zeid 2.5%, Bio Arc 6.0 %, Lemongrass and Thyme oil were used as seed dressing and soil drenching to investigate their effect in reducing damping-off and root rot disease incidence under greenhouse conditions. All compounds reduced the avocado damping-off and root rot disease incidence and increased the percentage of survival plants comparing with untreated seeds or untreated soil. Seed dressing and soil drenching with fungicides (Topsin M70, Kema-Z, Rizolex–T and Dithane M-45) showed a superior effect which produced the highest level of the disease control with the highest number of survived plants.</span><span> Treatments with </span><span>biofungicides (Bio Zied and Bio Arc) gave moderate effect. Essential oils (Thyme and Lemongrass) and Salicylic acid reduced the disease and increased mean survival plants of avocado transplants. Best results were obtained when the compounds were applied as seed dressing</span>Mansoura University, Faculty of AgricultureJournal of Plant Protection and Pathology2090-36779920180901Detection of Phytoplasmas Associated with Tomato Witches’ Broom and Big Bud Disease in Egypt5875904388610.21608/jppp.2018.43886ENS. I.BehiryAgricultural Botany Department, Faculty of Agriculture Saba Basha, Alexandria University, Egypt.Journal Article20190805<span>Symptoms of witches’ broom were observed on tomato plants in some fields in Aswan governorate, Egypt. Molecular investigation was done with phytoplasma-detection primer P1/P7 to amplify a fragment of the 16S rRNA gene plus spacer region and beginning of 23Sr gene. RFLP analyses and sequencing of DNA fragments amplified by R16F2n/R2 primers indicated that these phytoplasmas are closely related and could be classified in the 16SrII group. Phylogenetic analyses of 41 accessions of 16S ribosomal RNA gene sequences of ‘<em>Candidatus</em> phytoplasmas’ comprising the strain from Egypt and representative strains from GenBank confirmed that the phytoplasma from tomato cluster with other strains all classified in subgroup 16SrII-D. Therefore, it could be useful to use the RFLP methodology in rapid and specific screenings of this phytoplasma presence in Egyptian tomato plants</span>Mansoura University, Faculty of AgricultureJournal of Plant Protection and Pathology2090-36779920180901Characterization of Aster Yellows Phytoplasma Affecting Cycas revoluta in Egypt5915944388710.21608/jppp.2018.43887ENI. S.BehiryAgricultural Botany Department, Faculty of Agriculture Saba Basha, Alexandria University, Alexandria, EgyptJournal Article20190805In 2015, Pale yellowing symptoms were observed on <em>Cycas revoluta</em> (family: Cycadaceae) leaves rachis and lamina grown as ornamental plant in gardens of the Alexandria Governorate, Egypt. Since the symptoms were suggestive the phytoplasma presence, samples were tested by PCR amplification of 16S rRNA gene nucleotide sequences using phytoplasma universal primers P1/P7 in direct, and in nested R16F2n/R16R2 PCR. RFLP-PCR product of the nested primer with <em>Mse</em>I restriction enzyme and phylogenetic analyses of 16S rRNA gene nucleotide sequences suggested that the phytoplasma of Aster yellows designated in 16SrI group. Various 16Sr groups of phytoplasmas, e.g. 16SrI, 16SrII, 16SrX, 16SrXII, and 16SrXVII, are recognized to be associated in abnormalities of ornamental plants in many regions.However, this study reports for the first time the incidence of 16SrI phytoplasma in association with aster yellows disease in <em>Cycas revoluta</em> grown in Egypt.Mansoura University, Faculty of AgricultureJournal of Plant Protection and Pathology2090-36779920180901Biology and Control of Indian Meal Moth, Plodia interpunctella (Hubner) (Lepidoptera: Pyralidae) Infesting Stored Date, Almond and Peanut Fruits5956004388810.21608/jppp.2018.43888ENW. K. M.El-ShafeiCentral lab.for Date Palm, Agric. Res. Centre (ARC), Giza, EgyptRasha A.ZinhoumPlant Prot. Res. Inst., Agric. Res. Center (ARC), Dokki, Giza, EgyptH. B. H.HussainPlant Prot. Res. Inst., Agric. Res. Center (ARC), Dokki, Giza, EgyptJournal Article20190805This study aimed to evaluate different diets effects on the biological parameters of <em>plodia interpunctella </em>(Hubner). Three hosts namely; date, almond and peanut. Results showed that the hatchability percentage, weight per larva, pupation percentage and adult emergence percentage were the highest on almonds diet. Incubation period, generation time and life cycle were shortest in almonds. Also data indicated that almonds were the most preferred host. The efficacy of three microwave powers was investigated against two <em>plodia interpunctella</em> stages. The result cleared that the increase in microwave power and exposure times caused an increase in eggs and larvae reduction rate % and accordingly the microwave power more effective in larvae than egg stage. Also, results showed that there is no harmful effect on the chemical fruits characteristics under microwave treatment.Mansoura University, Faculty of AgricultureJournal of Plant Protection and Pathology2090-36779920180901Population Density, Food Consumption and Damage Caused by the Land Snail Monacha cantiana to Some Vegetable Crops at Kafr El-Sheikh Governorate6016044388910.21608/jppp.2018.43889ENWafaa A.ShahawyPlant Protection Research Institute, Agricultural Research Centre, Giza, EgyptNadia M.MostafaPlant Protection Research Institute, Agricultural Research Centre, Giza, EgyptHend S.El-TahawePlant Protection Research Institute, Agricultural Research Centre, Giza, EgyptJournal Article20190805The land snail <em>Monacha cantiana</em> is one of the most serious pests especially at north and east of Delta. It causes considerable damage to the majority of crops at Kafr El-Sheikh Governorate. In this study, a trial was carried out at Kafr El-Sheikh Governorate on the predominant land snail <em>M. canntiana</em> to throw light on its population density, food consumption and damage caused by it under field and laboratory conditions. Trial was conducted on some vegetable crops i.e. parsley, lettuce, onion, garlic, pepper and Jews mallow showed that the highest number of snails was recorded in March for onion and pepper with 65.6 and 19.0 snails/½ m<sup>2</sup>, respectively and in April for parsley, lettuce, garlic and Jews mallow with 68.6, 81.6, 74.6 and 7.0 snails/½ m<sup>2</sup>, respectively during 2015 season. In general, <em>M. cantiana</em> recorded highest infestation levels during March and April as compared to May, but numbers of snails in 2016 season were clearly less than 2015 season. The mean consumption values of <em>M. cantiana </em>snails for fresh leaves of some vegetable plants under laboratory conditions were 0.546, 0.185, 0.320, 0.070 and 0.117 g/10 snails/5 days for lettuce, parsley, onion, garlic and pepper, respectively. It is clear that lettuce leaves were most favorite for snails, while garlic leaves were the least. Concerning determination the damage caused by <em>M. cantiana</em> to fresh lettuce leaves under field conditions, the obtained data showed that the reduction percentage in lettuce leaf area reached its maximum value (4.81%) in the middle of April, while the minimum value (1.17%) was in the end of the same month.Mansoura University, Faculty of AgricultureJournal of Plant Protection and Pathology2090-36779920180901Study of Infection Impact by Entamopathogenic Fungi on some Biochemical Contents in Haemolymph of Cotton Leaf Worm, Spodoptera littoralis (Boisduval)6056104389110.21608/jppp.2018.43891ENS. S.El-BadawyPlant Prot. Res. Inst., Agric. Res. Center, Dokki, Giza, Egypt.Sahar S.AliPlant Prot. Res. Inst., Agric. Res. Center, Dokki, Giza, Egypt.A. A.El-HefnyPlant Prot. Res. Inst., Agric. Res. Center, Dokki, Giza, Egypt.Gamila A. M.HeikalPlant Prot. Res. Inst., Agric. Res. Center, Dokki, Giza, Egypt.Journal Article20190805Carbohydrates, lipids and proteins contents were evaluated in 5<sup>th </sup>larval instar haemolymph of <em>Spodoptera </em><em>littoralis </em>(Boisd.) that treated with four isolates of entomopathogenic fungi <em>Beauveria bassiana</em>, <em>Metarhizium anisopliae, Paecilomyces lilacinus </em>and <em>Lecanicillium antillanum </em>at 1<sup>st</sup>, 2<sup>nd</sup>, 3<sup>rd</sup> and 4<sup>th</sup> days. Generally, data in present work cleared that, there was significant decrease in haemolymph carbohydrates of larvae injected with the entomopathogenic fungi in the three time intervals (1<sup>st</sup>, 2<sup>nd</sup>and 3<sup>rd</sup>) under investigation compared to control. The concentration level of protein content of larvae injected with the fungi significantly increased as compared with control insects in (1<sup>st</sup>) day, then there is no significant change in the content after 2<sup>nd</sup> and 3<sup>rd</sup> day except increasing was noticed with the two fungi (<em>B. bassiana </em>and <em>L. antillanum</em>).There were significant increases of carbohydrates and protein contents observed in the last day (4<sup>th</sup> day) due to effect of fungal infection. Significant increases of lipid content was noticed in the 5<sup>th</sup>instar cotton leaf worm larvae inoculated with the four tested fungi from 1<sup>st</sup>to 4<sup>th</sup> day. The overall results indicated that total <em>S. littoralis </em>larvae proteins, carbohydrates and lipids contents vary significantly during infection of the tested fungi at the four time intervals (1<sup>st</sup>, 2<sup>nd</sup> 3<sup>rd</sup>and4<sup>th</sup> days) and this tend to dramatically changes in the composition of haemolymph, thus abnormal growth and development of insect caused.Mansoura University, Faculty of AgricultureJournal of Plant Protection and Pathology2090-36779920180901Checklist of Subfamilies Aleocharinae, Euaesthetinae, Omaliinae and Proteininae Belonging to Family Staphylinidae: Coleoptera in Egypt6116194389310.21608/jppp.2018.43893ENE. E. A. M.BibarsPlant protection Res. Inst. Agriculture Res. Cent., Giza, EgyptJournal Article20190805A systematic checklist of the Egyptian species of subfamilies Aleocharinae, Euaesthetinae, Omaliinae and Proteininae (Staphylinidae: Coleoptera) is presented. Subfamily Aleocharinae consists of 84 species belonging to 9 tribes, 33 genera and 23 subgenera, the second subfamily includes one species, the third subfamily contains two tribes, three genera and four species and the last subfamily includes two species belonging to two genera. Synonymys and distribution of all species are given. The checklist is arranged according to species membership in higher – level taxa, based on the most recent literatures of family Staphylinidae.