BIOCHEMICAL STUDIES ON THE EFFECT OF Metarhizium anisopliae VAR acridum INFECTION IN THE DESERT LOCUST, Schistocerca gregaria FORSKAL

Document Type : Original Article

Authors

1 Department of Entomology & Pesticides, Fac. Agric., Cairo University

2 Department of Locust and Grasshopper, Plant Protection Research Institute, Agriculture Research Center.

Abstract

Fifth instar nymphs of the desert locust Schistocerca gregaria Forskal were topically treated with 5µl of an oil-based spore suspension of the entomopathogenic fungus Metarhizium anisopliae var acridum. Haemolymph samples were collected from both control and treated insects every 24 hours starting from day 2 after the treatment.
         Estimation of total protein, total carbohydrate, total lipids and cholesterol were carried out. The results showed that the haemolymph content of the treated insects was highly affected and all the studied parameters were lower than in the control insects the decrease in the haemolymph content increased by increasing the post-inoculation period.

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