EFFECT OF TEMPERATURE ON EFFICACY OF Beauveria bassiana VUILLEMIN AND Metarhizium anisopliae VAR.ACRIDIUM GAMS AND ROZSPAL AGAINST THE DESERT LOCUST, Schistocerca gregaria (FORSKAL).

Document Type : Original Article

Authors

1 Plant protection Department, Faculty of Agriculture, Ain Shams University

2 Locust and Grasshopper Research Department, Plant protection Research Institute. Agriculture Research center.

Abstract

The influence of temperature on efficacy of Beauveria bassiana and Metarhizium anisopliae against the desert locust, Schistocerca gregaria (Forskal), were investigated in the laboratory. Fifth nymphal instar was inoculated by 1.5 ×103 spores/nymph by topical application using micropipette under the pronotum, at 50% RH. B.bassiana caused mortality for insects at temperature between 22-31°C, and 25°C was the optimum that caused more rapid death and the LT50 was 5.3 days. In contrast M. anisopliae has wide range of temperature that can caused death until 37°C, but 28-31°C was the optimum temperature and the LT50 was 5 days. At this optimums temperature, four doses (1.5×102, 1.5×103, 1.5×104, and 1.5×105 spore/nymph)were used against 4th and 5th nymphal instars of the desert locust. For 4th nymphal instar, It' was  obvious that M. anisopliae caused rapid mortality among infected 4th instar nymph comparing with B. basiana at all the tested doses except incase of dose1.5×102 spore /nymph, where treated nymph with B. bassiana died faster than those treated with M. anisopliae. While for the 5th nymphal instar, It' was clear that M. anisopliae caused significant rapid mortality comparing with B. bassiana in case of dose1.5×102spore/nymph. While B. bassiana caused significant rapid mortality than M. anisopliae in case dose1.5×104.

Keywords

Journal of Plant Protection and Pathology
Volume 34, Issue 6
June 2009
Pages 6891-6902

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