BIOCHEMICAL STUDIES OF Bacillus thuringiensis AND Spinosad ON THE CARBOHYDRATES HYDROLYZING ENZYMES AND PHOSPHATASE ENZYMES OF COTTON LEAF WORM, Spodoptera littoralis WITH SPECIAL COMPARISON OF PYRETHROID COMPOUND

Salama., Z. A.; Ebied., A. A.; Ahmad., H. A.; El Sheikh., T. A.

doi:10.21608/jppp.2007.219705

BIOCHEMICAL STUDIES OF Bacillus thuringiensis AND Spinosad ON THE CARBOHYDRATES HYDROLYZING ENZYMES AND PHOSPHATASE ENZYMES OF COTTON LEAF WORM, Spodoptera littoralis WITH SPECIAL COMPARISON OF PYRETHROID COMPOUND

Document Type : Original Article

Authors

Faculty of Science Mansoura University

10.21608/jppp.2007.219705

Abstract

This investigation Show the effect of the three tested compounds on the activity of Haemolymph acid and alkaline phosphatase of 5^th instar larvae of S. littoralis treated as 4^th instar larvae with the LC₅₀ levels of Spinosad, B. thuringiensis and Cypermethrin. The results indicated that the acid phosphatase activity was insignificantly increased by about 2.55% more than the control in case of treatment with B. thuringiensis. On the other hand, Spinosad and Cypermethrin insignificantly decreased the activity of acid phosphatase.The application of the three tested materials to the 4^th instar larvae of s. littoralis had changed the carbohydrate hydrolyzing enzymes as indicated that there was a large significant increase in amylase activity of 5^th instar larvae of S. littoralis in case of Spinosad treatment; and treatment with Cypermethrin significantly decreased the amylase activity, whereas treatment with B. thuringiensis significantly decreased in compared to control. Invertase activity was significantly decreased in case of all tested compound treatments compared to control. Trehalase activity was significantly decreased, compared to control for Spinosad treatments. Whereas, Cypermethrin and B. thuringiensis treatment insignificantly decreased trehalase activity compared to control.