SERO - AND MOLECULAR DIAGNOSIS FOR INCIDENCE OF Maize yellow stripe virus (MYSV) IN LOWER AND MIDDLE EGYPT

Abdel-Kader,, A. M.; Amin, Hala A.; Aboul-Ata, A. E.; El-Bolok, M.; Hussein, Amal M.; Ammar, E. D.

doi:10.21608/jppp.2007.220700

SERO - AND MOLECULAR DIAGNOSIS FOR INCIDENCE OF Maize yellow stripe virus (MYSV) IN LOWER AND MIDDLE EGYPT

Document Type : Original Article

Authors

¹ Dept. of Plant Virus and Phytoplasma Res., Plant Path. Res. Inst., ARC, P.O.Box, 12619, Giza, Egypt

² Dept. of Economic Entomology and Pesticides, Faculty of Agriculture, Cairo, University, Giza, Egypt

³ Molecular Biology Research Institute, Munofia University, Sadat City, Munofia, Egypt

10.21608/jppp.2007.220700

Abstract

Maize Yellow Stripe Virus (MYSV) is a leafhopper-borne Tenuivirus thathas induced some epidemics on maize plants earlier in Egypt. Field samples from maize plantations were taken during the summer and Nily growing seasons of 2004 and 2005 to estimate the occurrence of MYSV on maize in ten governorates of Middle and Lower Egypt. Disease severity and percentage of MYSV incidence were recorded. Visual examination of MYSV symptoms, serodiagnosis using DAC-ELISA, and sometimes molecular tools (IC/RT-PCR and hybridization) were used to detect the disease occurrence in maize leaf samples. Generally, MYSV occurrence was higher in Middle than in Lower Egypt. Also, it was higher in Nily than in summer plantations. However, MYSV incidence in the summer season of 2004 was higher than in Nily season of the same year. The epidemiology and transmission of this virus by Cicadulina chinai is discussed.

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