EFFECT OF AWILD TYPE AND THREE DIFFERENT MUTANTS OF CHITINOLYTIC BACTERIA, Bacillus thuringiensis BY U.V. IRRADIATION ON VIABILITY OF Meloidogyne incognita EGGS UNDER LABORATORY CONDITIONS

Abd- El Bary,, Nagwa A.; Eissa, M. F. M.; Yassin, M. Y.; Ismail, A. E.; Badr, U. M.; Soliman, Gaziea M.

doi:10.21608/jppp.2007.220987

EFFECT OF AWILD TYPE AND THREE DIFFERENT MUTANTS OF CHITINOLYTIC BACTERIA, Bacillus thuringiensis BY U.V. IRRADIATION ON VIABILITY OF Meloidogyne incognita EGGS UNDER LABORATORY CONDITIONS

Document Type : Original Article

Authors

¹ Agriculture Zoology and Nematology Dept, Fac. of Agric., Cairo University, Egypt.

² Nematology Laboratory, Plant Pathology Dept, National Research Centre, Dokki Cairo, Egypt.

³ Genetic and Cytology Dept, N R C, Dokki Cairo, Egypt

10.21608/jppp.2007.220987

Abstract

The nematicidal activity of the wild type and three mutants namely (24, 10, 32 ) of the chitinolytic bacteria, Bacillus thuringiensis was evaluated against eggs of the root - knot nematode, Meloidogyne incognita, using U.V. irradiation as a safer and environment friendly control, alternate of chemical nematicides under laboratory conditions at 25 ± 5 ºC.
Results, generally indicated that the mutant no. 24 is more effective on the nematode eggs followed by mutant no.10, then mutant no. 32 as compared to the wild type which achieved the lowest mortality. These values ranged from (90.8 to 96.6 % ), (79.8 to 89.9 %), ( 70.2 to 79.5 %), (46.3 to 68.8 %); respectively compared to 2.7% in untreated control after 96 he time. There were positive relation - ship between the percentage of mortality and the chitinase production from mutants, the production evaluated 385 %, 350 % and 300 %; respectively.

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