Abdel - Aal,, A., Mariy, F., Youssef, L. (2007). BIOLOGICAL AND HISTOLOGICAL EFFECTS OF SPINOSAD ON spodoptera littoralis (BOISD.).. Journal of Plant Protection and Pathology, 32(12), 10497-10510. doi: 10.21608/jppp.2007.221193
Aziza E. Abdel - Aal,; Faiza M. A. Mariy; L. A. Youssef. "BIOLOGICAL AND HISTOLOGICAL EFFECTS OF SPINOSAD ON spodoptera littoralis (BOISD.).". Journal of Plant Protection and Pathology, 32, 12, 2007, 10497-10510. doi: 10.21608/jppp.2007.221193
Abdel - Aal,, A., Mariy, F., Youssef, L. (2007). 'BIOLOGICAL AND HISTOLOGICAL EFFECTS OF SPINOSAD ON spodoptera littoralis (BOISD.).', Journal of Plant Protection and Pathology, 32(12), pp. 10497-10510. doi: 10.21608/jppp.2007.221193
Abdel - Aal,, A., Mariy, F., Youssef, L. BIOLOGICAL AND HISTOLOGICAL EFFECTS OF SPINOSAD ON spodoptera littoralis (BOISD.).. Journal of Plant Protection and Pathology, 2007; 32(12): 10497-10510. doi: 10.21608/jppp.2007.221193
BIOLOGICAL AND HISTOLOGICAL EFFECTS OF SPINOSAD ON spodoptera littoralis (BOISD.).
1Plant Protection Research Institute, Agric. Res. Center, Dokki,Giza, Egypt.
2Plant Protection Dept., Fac. of Agric., Ain Shams Univ., Shubra El-Keima, Cairo, Egypt.
Abstract
The efficiency of Spinosad was evaluated on larvae of the Egyption cotton leaf worm, Spodoptera littoralis (Boisd): (Lepidoptera: Noctuidae). Second instar larvae were more sensitive to Spinosad than 4th instar larvae, as denoted by the determined LC50 which was 5.73 and 7.83 ppm for 2nd and 4th instars, respectively. Meanwhile, LC90 was 37.15 and 43.49 ppm for the respectively mentioned instars compared to their control. The duration of treated larvae was lengthened when either instars larvae were treated as compared to their control. Furthermore, percentage of pupation was reduced than the control by 51.03 and 49.5% when 2nd and 4th instar larvae were treated with LC50 of Spinosad, respectively. Adult emergence was insignificantly affected as it was less than the control by 5.3 and 4.1% to the respective mentioned treated larval instars. The number of laid eggs was insignificantly affected; however, percentage hatchability was markedly reduced. The total protein content in larvae treated as 4th instar showed a gradual increase from 2nd and 6th days after treatment as it was 34.79- 35.18 mg respectively, compared with 25.8 to 26.5 mg in control. The midgut tissue of 5th instar larvae treated in the 4th instar larvae with LC50 of Spinosad showed some alternations and several aberration in some cell organelle such mitochondria, nucleolus, microvilli and golgi apparatus as observed by light and electron microscope.
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