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Radwan,, E. (2006). GENETIC DIFFERENCES BETWEEN SUSCEPTIBLE, RESISTANT AND FIELD STRAINS OF THE COWPEA APHID, Aphis craccivora (Koch). Journal of Plant Protection and Pathology, 31(6), 3929-3937. doi: 10.21608/jppp.2006.235226
Eman M. M. Radwan,. "GENETIC DIFFERENCES BETWEEN SUSCEPTIBLE, RESISTANT AND FIELD STRAINS OF THE COWPEA APHID, Aphis craccivora (Koch)". Journal of Plant Protection and Pathology, 31, 6, 2006, 3929-3937. doi: 10.21608/jppp.2006.235226
Radwan,, E. (2006). 'GENETIC DIFFERENCES BETWEEN SUSCEPTIBLE, RESISTANT AND FIELD STRAINS OF THE COWPEA APHID, Aphis craccivora (Koch)', Journal of Plant Protection and Pathology, 31(6), pp. 3929-3937. doi: 10.21608/jppp.2006.235226
Radwan,, E. GENETIC DIFFERENCES BETWEEN SUSCEPTIBLE, RESISTANT AND FIELD STRAINS OF THE COWPEA APHID, Aphis craccivora (Koch). Journal of Plant Protection and Pathology, 2006; 31(6): 3929-3937. doi: 10.21608/jppp.2006.235226

GENETIC DIFFERENCES BETWEEN SUSCEPTIBLE, RESISTANT AND FIELD STRAINS OF THE COWPEA APHID, Aphis craccivora (Koch)

Article 2, Volume 31, Issue 6, May and June 2006, Page 3929-3937  XML PDF (2.66 MB)
Document Type: Original Article
DOI: 10.21608/jppp.2006.235226
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Author
Eman M. M. Radwan,
Central Agric. Pesticides Lab., A~ric. Res. Center, Dokki Giza.
Abstract
Random Ampilified polymorphic. DNA Polymerase Chain Reaction (RAPD-
PCR) method one from several approaches of assaying genetic variation in molecular
biology science. This analysis was performed using a battery of five primers to
evaluate the mutagenic effect among the cowpea aphid, Aphis craccivora resistant
strain of dinotefuran insecticide (neonicotcnoid) compared with field and laboratory
susceptible strains. The five primers (A-14, 16, 17, 18 and 19) yielded 96 fragments,
primer A -18 detected 57 % difference between genome of field and resistant aphids.
The same primer gave 33% variation between susceptible and resistant aphids
genome. The highest degree of difference (60%) between the field and susceptible
populations DNA sequence was revealed by primer A-14. The high diversity in
genome of field and dinotefuran resistant strains compared with the susceptible strain
may be explain the activity of dinotefuran against field population of cowpea aphid and
. support the valuble application of this insecticide in control of A.croccivora ( Koch ).
Keywords
The cowpea aphid; dinotefuran (neonicotinoid insecticide); RAPD; PCR
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