KARYOTYPING CHARACTERlZATlON OF A CONTINUOUS NEW CELL LINE FROM PUPAE OVARIES OF EGYPTIAN COTTON LEAF WORM Spodoptera littoralis (SL-OMl) ADAPTED 0N 27°C

Khamiss, Omaimau; El-Helaly’, Alexandra; Ammara, El-D.; Abol—Ela, S.

doi:10.21608/jppp.2005.238944

KARYOTYPING CHARACTERlZATlON OF A CONTINUOUS NEW CELL LINE FROM PUPAE OVARIES OF EGYPTIAN COTTON LEAF WORM Spodoptera littoralis (SL-OMl) ADAPTED 0N 27°C

Document Type : Original Article

Authors

¹ Center of Virology, Faculty of Agriculture-RD. Cairo Univ., Egypt.

² Genetic Engineering and Biotechnology inst, Menofeya University.

³ Economic Entomology Dept. Faculty of Agric., Cairo Unlv., Egypt.

10.21608/jppp.2005.238944

Abstract

A new ovarian cell line was established from pupae of Egyptian cotton leaf
worm Spodoptera iiitoralis. cells were adapted at 270 and characterized at the
morphological. growth. and keryotyping . The number of chromosomes was counted
and compared with reference cell lines Sf9. Sl96 and with the number of
chromosomes on eggs (in viva) oi the same source of insect. Cell doubling time was
24hr. Chromosomes were morphological typical to those described from other species
of Lepidopterous. Chromosomes number was counted on 5? metaphases for the new
cell line (SL omi) and it was ranged between 4-203 Chromosomes. As for 58
metaphases were prepared from eggs. the counted chromosomal number was ranged
between 4— 24 on 57 metaphases and one only metaphase had 46 chromosomes.
Obtained results from eggs chromosomes preparation and Si omi was different from
those prepared from Sf9, which ranged between Bland 263 in 68 metaphase (Ref).