Moustafa,, M., Helal, M., EI- Dakar, H. (2004). EFFECT OF LASER IRRADIATION ON SEED BORNE FUNGI OF RICE. Journal of Plant Protection and Pathology, 29(3), 1495-1503. doi: 10.21608/jppp.2004.239520
M. S. H. Moustafa,; M. R. Helal; H. A. M. EI- Dakar. "EFFECT OF LASER IRRADIATION ON SEED BORNE FUNGI OF RICE". Journal of Plant Protection and Pathology, 29, 3, 2004, 1495-1503. doi: 10.21608/jppp.2004.239520
Moustafa,, M., Helal, M., EI- Dakar, H. (2004). 'EFFECT OF LASER IRRADIATION ON SEED BORNE FUNGI OF RICE', Journal of Plant Protection and Pathology, 29(3), pp. 1495-1503. doi: 10.21608/jppp.2004.239520
Moustafa,, M., Helal, M., EI- Dakar, H. EFFECT OF LASER IRRADIATION ON SEED BORNE FUNGI OF RICE. Journal of Plant Protection and Pathology, 2004; 29(3): 1495-1503. doi: 10.21608/jppp.2004.239520
EFFECT OF LASER IRRADIATION ON SEED BORNE FUNGI OF RICE
Plant Patholoqy Research Institute, Agricultural Research Center, Giza, Egypt.
Abstract
In this work, an apparatus for irradiation of the rice seeds with LASER beam. was developed .This apparatus design is described.
Seeds of rice (Giza 159 cv.) were irradiated with LASER beam for different periods (0, 15, 30, 45 and 60 s.). Irradiation of rice seeds resulted in increasing of the seed germination percent; however, prolonging the irradiation period to 60 s. had lethal effect on the seeds. Isolation of different seed-borne fungi from irradiated and non-irradiated rice seeds showed obvious differences either in total number or number of each fungus isolated from the different treatments. Increasing the irradiation period was correlated with decreasing of the seed-borne fungi. While 41 isolates were obtained from untreated seeds, only 26 isolates were obtained from seeds treated with LASER beam for 15 s. compared with 3 isolates obtained from seeds treated for 30 s. Irradiation of the seeds for 45 s. resulted in complete disinfections of the seeds. Trichoderma sp. and Peniclilium sp. were isolated from the surface of the seeds. Trichoderma isolates were more sensitive to the irradiation than the Penicillium isolates.
Isolation from seed coat showed that the dominant fungi were Pyricularia oryzae and Helminthosporium sp. while Fusarium sp. was found in the endosperm. Irradiation period of 30 or 45 s. resulted in complete elimination of these fungi.
Under greenhouse conditions the plants, emerged from irradiated and non-irradiated seeds, showed different disease severity, which was correlated negatively with prolonging of the irradiation period: in contrary, the height of plants and fresh weight of the foliage and roots were correlated positively with prolonging of the irradiation ceriod
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