TECHNQUE OF ROOT-KNOT NEMATODE, Me/oidogyne javanica

Bunt's technique (1975), is used for screening nematicides. A simple
procedure has been developed using some modification that is suitable for rearing
root-knot nematode with minimal chemicals, labors and greenhouse space as
required. The different stages of nematode that used as initial inocula (egg-masses or
second stage juveniles) succeeded in complement their life cycle and reproduction
under irrigation with water or nutrient solution (O.B gm ammonium sulfate 20.5%, 0.1
gm potassium sulfate 47%, and 0.6 gm calcium superphosphate 15% I liter of water).
Fertilizer treatment suppressed gall formation in case of inocula with egg-
masses or juveniles whereas, such reduction was higher with plants inoculated with
egg-masses than these inocula with juveniles. In contrast, fertilizers play an important
role in increasing number of laying female. These findings are associated with
increasing shoot moisture and decreasing root moisture. The results showed the
highest juvenile yield number of egg-massesf plant and percentage of egg-massfgall
ratio in case of inoclum with juveniles under fertilizer conditions. This work needs
further investigations.