Aly,, N., Abd - EI Rahman, S. (2004). EFFECT OF CERTAIN PESTICIDES ON SOME ENZYMES OF CHICK EMBRYOS. Journal of Plant Protection and Pathology, 29(11), 6605-6616. doi: 10.21608/jppp.2004.240062
Nagat M. Aly,; S. M. Abd - EI Rahman. "EFFECT OF CERTAIN PESTICIDES ON SOME ENZYMES OF CHICK EMBRYOS". Journal of Plant Protection and Pathology, 29, 11, 2004, 6605-6616. doi: 10.21608/jppp.2004.240062
Aly,, N., Abd - EI Rahman, S. (2004). 'EFFECT OF CERTAIN PESTICIDES ON SOME ENZYMES OF CHICK EMBRYOS', Journal of Plant Protection and Pathology, 29(11), pp. 6605-6616. doi: 10.21608/jppp.2004.240062
Aly,, N., Abd - EI Rahman, S. EFFECT OF CERTAIN PESTICIDES ON SOME ENZYMES OF CHICK EMBRYOS. Journal of Plant Protection and Pathology, 2004; 29(11): 6605-6616. doi: 10.21608/jppp.2004.240062
EFFECT OF CERTAIN PESTICIDES ON SOME ENZYMES OF CHICK EMBRYOS
Central Agric. Pesticide Laboratory, Agric. Res. Center, Sabhia, Alexandria, Egypt.
Abstract
Effects of Chlorpyrifos 48 % EC ( Dursban & Pestban ) and Profenofos 72 % EC (Selecron & Ictacron) at two sublethal concentrations (1/100thand 1I10th of the field recommended rate ) on some serum , brain and liver enzymes as well as total protein from 18 and 21 days old - chick embryos (hatching) were determined. In general, Dursban and Pestban at the two concentrations significantly decreased brain acetylcholinesterase (AChE) either at 18 or 21 days old chick embryos, except Dursban in 18 days at the low concentration. On the oiher hand, Selecron and Ictacron at the two concentrations caused insignificant inhibition in brain AChE activity after 18 and 21 days compared with the control groups.
Serum alanine amino transferase (AL T) specific activity was significantly increased with Chlorpyrifos and Profenofos at the used concentrations. On the contrary, Chlorpyrifos from the two formulations at the high concentration Significantly inhibited the serum aspartate amino tranferase (AST). There were no differences between the two formulations of Profenofos on the serum amino transferases (AL T & AST).
The results also revealed that the activity of liver acid phosphatase (AcP) in 18 days old chick embryos was insignificantly inhibited at the two concentrations of Chlorpyrifos ( Dursban & Pestban ). There were no Significant differences between Selecron and Ictacron on the liver enzyme activity at 18 days. Chlorpyrifos and Profenofos were caused significant inhibition of 21 days old chick embryo liver (AcP) activities from the two formulations, except Selecron at the high concentration.
The activity of liver alkaline phosphatase (AlP) in the 18 days old - chick embryos was decreased insignificantly at the two concentrations of Dursban, in contrast, the activity was increased by increasing the concentration of Pestban, Selecron and Ictacron. On 21 days old - chick embryos, Chlorpyrifos (Dursban & Pestban) caused significant decrease of liver AlP activity at the two concentrations. Selecron at the two concentrations had insignificant effect on serum AlP activity, but Ictacron caused significant activation of serum AlP activity at the high concentration.
There were insignificant changes in the serum total protein between the treated groups with Dursban, Pestban, Selecron, Ictacron and control groups.