Abd El-All, S., Zedan, H., K.hidr, F., Sanad, A. (2003). PRELIMINARY PHYTOCHEMICAL SCREENNING SEPARATION AND IDENTIFICATION OF CERTAIN ACTIVE COMPONENTS FROM SOME PLANT EXTRACTS.. Journal of Plant Protection and Pathology, 28(9), 7025-7042. doi: 10.21608/jppp.2003.252491
S. M. Abd El-All; H. A. Zedan; Fatma K. K.hidr; A. E. Sanad. "PRELIMINARY PHYTOCHEMICAL SCREENNING SEPARATION AND IDENTIFICATION OF CERTAIN ACTIVE COMPONENTS FROM SOME PLANT EXTRACTS.". Journal of Plant Protection and Pathology, 28, 9, 2003, 7025-7042. doi: 10.21608/jppp.2003.252491
Abd El-All, S., Zedan, H., K.hidr, F., Sanad, A. (2003). 'PRELIMINARY PHYTOCHEMICAL SCREENNING SEPARATION AND IDENTIFICATION OF CERTAIN ACTIVE COMPONENTS FROM SOME PLANT EXTRACTS.', Journal of Plant Protection and Pathology, 28(9), pp. 7025-7042. doi: 10.21608/jppp.2003.252491
Abd El-All, S., Zedan, H., K.hidr, F., Sanad, A. PRELIMINARY PHYTOCHEMICAL SCREENNING SEPARATION AND IDENTIFICATION OF CERTAIN ACTIVE COMPONENTS FROM SOME PLANT EXTRACTS.. Journal of Plant Protection and Pathology, 2003; 28(9): 7025-7042. doi: 10.21608/jppp.2003.252491
PRELIMINARY PHYTOCHEMICAL SCREENNING SEPARATION AND IDENTIFICATION OF CERTAIN ACTIVE COMPONENTS FROM SOME PLANT EXTRACTS.
1Plant Protection Dept., Faculty of Agric., Al-Azhar Univ. Assiut, Egypt.
2Plant Protection Res. Inst. Agric.Res. Center, Dokki, Giza, Egypt.
Abstract
The phytochemical screening of the active constituents of six wild plants were studied in leaves and seeds of both black pepper khella, camphor, geranium, datura and oshar plant extracted using different organic solvents. On the other hand, plant constituents of camphor, datora, oshar and geranium extracted only of both leaves and seeds were separated and identified by using the most proper techniques. Data indicated that nine phytochemical components were separated in various amounts due to plant species and plant organs as well as solvents used in extraction. In general, sterols & triterpenes, phenolic glycosides, alkaloids and carbohydrates & glycosides were found in relatively larger amounts followed by anthraquinone glycosides, tannins which were found in large amounts followed by saponin, flavonoids and cardiac glycosides which were found in traces. TLC of the crude plant extracts reveald the separation of five spols from granuim hexane extract, three spots from both datura and oshar ethanol, (five and one spots) from camphor (leaves and seeds) hexane and (five and three spots) from camphor (leaves and seeds ) ethanol crude extracts with R f values of [ (0.11, 0.33, 0.57, 0.71 and 0.89) and (0.29, 0.64 and 0.79 ) and ( 0.28, 0.71 and 0.85)], [ (0.27, 0.50, 0.70, 0.80 and 0.97 ) and (0.97), ( 0.06, 0.16, 0.55, 0.84 and 0.97 ) and ( 0.27, 0.33 and 0.93 )] respectively. The function groups of the isolated fractions were also identified using IR spectral analysis.
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