Impact of Chemical Composition and Molecular Diversity of Sugar Beet Cultivars on Scrobipalpa ocellatella Boyd (Lepidoptera: Gelechiidae) and Cassida vittata Vill (Coleoptera: Chrysomelidae)

Kandil, Ragab Sebaita; Fayed, A. M.; El-Dessouki, W. A.

doi:10.21608/jppp.2023.241516.1186

Impact of Chemical Composition and Molecular Diversity of Sugar Beet Cultivars on Scrobipalpa ocellatella Boyd (Lepidoptera: Gelechiidae) and Cassida vittata Vill (Coleoptera: Chrysomelidae)

Document Type : Original Article

Authors

¹ Field Crop Pests Research Department, Plant Protection Research Institute, Agricultural Research Center, Dokki, Giza, Egypt.

² Molecular Biology Dept., Genetic Engineering and Biotechnology Res. Institute, Sadat University, Egypt.

³ Department of Plant Protection, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt.

10.21608/jppp.2023.241516.1186

Abstract

The field experiments were carried out at the experimental farm of Sakha Agricultural Research Station, Kafr El-Sheikh Governorate, Egypt to study the influence of both chemical composition and molecular variations of sugar beet cultivars on the population of Scrobipalpa ocellatella and Cassidavittata in 2020-2021 and 2021-2022 seasons. Results showed that Celnne cultivar harbored higher population of S. ocellatella (16.50 and 18.67 larvae/ 5 plants) in February and C. vittata (54.87 and 55.40 larvae and adults/ 5 plants) in March, with significant differences, than Heliospoly cultivar during the two investigated seasons, respectively. These differences may be due to the direct proportion between protein content and insect populations. A maximum standard of DNA polymorphism was found out by RAPD-PCR technique for both cultivars appearance certain positive and negative markers linked with plant afford to insect offensive. The RAPD-PCR techniqueof both cultivars revealed that Heliospoly was more resistant; while Celnne was more sensitive to these insects. The present study could be utilized by breeders and entomologists to develop sugar beet cultivars resistant to the two insects.

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