Impact of Chemical Composition and Molecular Diversity of Sugar Beet Cultivars on Scrobipalpa ocellatella Boyd (Lepidoptera: Gelechiidae) and Cassida vittata Vill (Coleoptera: Chrysomelidae)

Kandil, Ragab Sebaita; Fayed, A. M.; El-Dessouki, W. A.

doi:10.21608/jppp.2023.241516.1186

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	Impact of Chemical Composition and Molecular Diversity of Sugar Beet Cultivars on Scrobipalpa ocellatella Boyd (Lepidoptera: Gelechiidae) and Cassida vittata Vill (Coleoptera: Chrysomelidae)
Article 2, Volume 14, Issue 11, November 2023, Page 353-358 PDF (734.09 K)
Document Type: Original Article
DOI: 10.21608/jppp.2023.241516.1186
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Authors
Ragab Sebaita Kandil ¹; A. M. Fayed²; W. A. El-Dessouki³
¹Field Crop Pests Research Department, Plant Protection Research Institute, Agricultural Research Center, Dokki, Giza, Egypt.
²Molecular Biology Dept., Genetic Engineering and Biotechnology Res. Institute, Sadat University, Egypt.
³Department of Plant Protection, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt.
Abstract
The field experiments were carried out at the experimental farm of Sakha Agricultural Research Station, Kafr El-Sheikh Governorate, Egypt to study the influence of both chemical composition and molecular variations of sugar beet cultivars on the population of Scrobipalpa ocellatella and Cassidavittata in 2020-2021 and 2021-2022 seasons. Results showed that Celnne cultivar harbored higher population of S. ocellatella (16.50 and 18.67 larvae/ 5 plants) in February and C. vittata (54.87 and 55.40 larvae and adults/ 5 plants) in March, with significant differences, than Heliospoly cultivar during the two investigated seasons, respectively. These differences may be due to the direct proportion between protein content and insect populations. A maximum standard of DNA polymorphism was found out by RAPD-PCR technique for both cultivars appearance certain positive and negative markers linked with plant afford to insect offensive. The RAPD-PCR techniqueof both cultivars revealed that Heliospoly was more resistant; while Celnne was more sensitive to these insects. The present study could be utilized by breeders and entomologists to develop sugar beet cultivars resistant to the two insects.
Keywords
Beta vulgaris; Sugar beet moth; Sugar beet beetle; Molecular markers


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