El-Gendy, R., El-Shafiey, S. (2018). Eco-Friendly Control Strategies of Green Stink Bug, Nezara viridula L. (Hemiptera: Pentatomidae): Repellency and Toxicity Effects of Callistemon citrinus, Bottle Brush Essential Oil. Journal of Plant Protection and Pathology, 9(12), 807-813. doi: 10.21608/jppp.2018.44071
Rehab M. El-Gendy; Samah N. El-Shafiey. "Eco-Friendly Control Strategies of Green Stink Bug, Nezara viridula L. (Hemiptera: Pentatomidae): Repellency and Toxicity Effects of Callistemon citrinus, Bottle Brush Essential Oil". Journal of Plant Protection and Pathology, 9, 12, 2018, 807-813. doi: 10.21608/jppp.2018.44071
El-Gendy, R., El-Shafiey, S. (2018). 'Eco-Friendly Control Strategies of Green Stink Bug, Nezara viridula L. (Hemiptera: Pentatomidae): Repellency and Toxicity Effects of Callistemon citrinus, Bottle Brush Essential Oil', Journal of Plant Protection and Pathology, 9(12), pp. 807-813. doi: 10.21608/jppp.2018.44071
El-Gendy, R., El-Shafiey, S. Eco-Friendly Control Strategies of Green Stink Bug, Nezara viridula L. (Hemiptera: Pentatomidae): Repellency and Toxicity Effects of Callistemon citrinus, Bottle Brush Essential Oil. Journal of Plant Protection and Pathology, 2018; 9(12): 807-813. doi: 10.21608/jppp.2018.44071
Eco-Friendly Control Strategies of Green Stink Bug, Nezara viridula L. (Hemiptera: Pentatomidae): Repellency and Toxicity Effects of Callistemon citrinus, Bottle Brush Essential Oil
Plant Protection Research Institute, Agriculture Research Center, Dokki, Giza
Abstract
The bioactivity of Callistemon citrinus leaves essential oil against green stink bug, Nezara viridula was evaluated as alternative eco-friendly pesticide. The tested essential oil have high insecticidal activity toN. viridula adult where, the lethal concentrations of LC10, LC25 and LC50 were recorded (105.22, 536.80 and 3274.72 ppm), respectively. The repellent effects on N. viridula adult of the three tested concentrations were 48.01, 56.35 and 81.33 % after one hour of exposure time, respectively. The chemical composition analysis of bottle brush essential oil by GC/MS revealed 24 compounds represented 99.47% of the whole amount oil. The major constituent 1,8- cineole represented (73.81%) followed by α pinene (13.13%), D-limonene (5.50%), β-pinene (2.49%) and α-Terpineol (1.10%) assumed 96.03% of the total oil composition, while the minor components were 3.97%. Treatment with all concentrations of C. citrinus essential oil exhibited some physiological defects that cleared in the reduction in total soluble protein levels and elevation in carbohydrate hydrolyzing enzymes, nonspecific estrases and acid & alkaline phosphatases activities. Moreover, the LC50 inhibited acetyl cholinesterase activity in treated adults. Therefore the oil proved high potency in controlling the N. viridula.
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