El-Sheikh, M., El-Shami, I. (2017). Isolation and Virulence of Beauveria bassiana (Bals.) Vuill. (Deuteromycotina: Hyphomycetes) Isolates from Hibernated Ostrinia nubilalis (Hb.) and Sesamia cretica Led. from Maize Stalks. Journal of Plant Protection and Pathology, 8(11), 549-553. doi: 10.21608/jppp.2017.46604
M. F. El-Sheikh; I. A. El-Shami. "Isolation and Virulence of Beauveria bassiana (Bals.) Vuill. (Deuteromycotina: Hyphomycetes) Isolates from Hibernated Ostrinia nubilalis (Hb.) and Sesamia cretica Led. from Maize Stalks". Journal of Plant Protection and Pathology, 8, 11, 2017, 549-553. doi: 10.21608/jppp.2017.46604
El-Sheikh, M., El-Shami, I. (2017). 'Isolation and Virulence of Beauveria bassiana (Bals.) Vuill. (Deuteromycotina: Hyphomycetes) Isolates from Hibernated Ostrinia nubilalis (Hb.) and Sesamia cretica Led. from Maize Stalks', Journal of Plant Protection and Pathology, 8(11), pp. 549-553. doi: 10.21608/jppp.2017.46604
El-Sheikh, M., El-Shami, I. Isolation and Virulence of Beauveria bassiana (Bals.) Vuill. (Deuteromycotina: Hyphomycetes) Isolates from Hibernated Ostrinia nubilalis (Hb.) and Sesamia cretica Led. from Maize Stalks. Journal of Plant Protection and Pathology, 2017; 8(11): 549-553. doi: 10.21608/jppp.2017.46604
Isolation and Virulence of Beauveria bassiana (Bals.) Vuill. (Deuteromycotina: Hyphomycetes) Isolates from Hibernated Ostrinia nubilalis (Hb.) and Sesamia cretica Led. from Maize Stalks
1Department of Plant Protection, Faculty of Agriculture, Tanta University, Egypt
2Plant Protection Research Institute, Agricultural Research Center
Abstract
The experiments were carried out at three locations in Gharbia governorate which heavily infested with corn borers larvae, Ostrinia nubilalis and Sesamia cretica, on maize stalks abandoned in the field, these locations were Tanta, El-Santa and Quator locations during the period from November to April in 2014/15 season. A proportion reduction in number of the two pests occurred during overwintering in maize stalks by entomopathogenic fungi, Beauveriabassiana isolates. The rate of reduction revealed 28.57, 6.74 and 10.41 % of O. nubilalis larvae at Tanta, El-Santa and Qutour, resepectively and 7.69 % in S. cretica larvae only in El-Santa. Seventeen fungi isolates of the fungus B. bassiana were found on O. nubilalis larvae at the three locations then the germination of fungi isolates was measured and found that the Bb-OLT 26 Jan isolate was the highest of conidia germinated then selected to bioassy study. The two fungi isolates Bb-OLT 26 and Bb-SLE 26 Jan were treated on O. nubilalis and S. cretica, respectively in labortatory conditions with concentrations of 104, 105, 106, 107 and 108 conidia/ml. Therefore the LC50 values were 1.23 x 105and 1.18 x 107 conidia/ml. for Bb-OLT 26 and Bb-SLE 26 Jan isolates, respectively. This results indicated that the entomopathogenic fungus, B. bassiana is an effective a natural pathogen causing a consideral mortality in the hibernated larvae of O. nubilalis which may be important in the natural regulation of the pest and encourage to apply it on the field populations at maize fields.
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