BIOCHEMICAL IMPACTS OF FUNGUS Metarihizium anisopliae ON THE ADULT FEMALES OF THE TWO SPOTTED SPIDER MITE Tetranychus urticae KOCH.

Mikhail, W. A.; Sobhy, H. M.; Nada, Maha S.; El- Naggar, M. E.; Mahmoud, Rania H.

doi:10.21608/jppp.2015.74945

BIOCHEMICAL IMPACTS OF FUNGUS Metarihizium anisopliae ON THE ADULT FEMALES OF THE TWO SPOTTED SPIDER MITE Tetranychus urticae KOCH.

Document Type : Original Article

Authors

¹ -Natural Resources Dept. ‚ Inst. African Research &Studies ‚Cairo Univ.‚Egypt.

² Natural Resources Dept. ‚ Inst. African Research &Studies ‚Cairo Univ.‚Egypt.

³ Plant Protection Research Institute‚ A.R.C.

10.21608/jppp.2015.74945

Abstract

The two-spotted spider mite Tetranychus urticae koch is a very destructive pest causing considerable loss to crops. Metarihizium anisopliae fungus strain is highly specific biopesticide to mites. The effect of fungus M. anisopliae was studied on the major components of the two-spotted spider mite, adult females reared in laboratory and treated by LC₂₅ (4 x 10⁶ spores/ml). Mite adult females were susceptible to M. anisopliae with high mortality rate recorded. Total mites protein, carbohydrates and lipids were declined in the treatment. . The effect of M.anisopliae on proteins level in the mycosed extract homogenate appeared decreasing than the non-mycosed during the period of experiment. The means of total protiens are 149‚148and85µg⁄1000 individuals after 24‚48 and 72hrs respectively‚compared with165 µg⁄1000 individuals in untreated control. Effect of the M.anisopliae on the level of the carbohydrates decreased in the treated extract homogenate than the untreated one during the period of experiment. The mean of total carbohydrates at 24‚48and 72 hrs after treatment were 96‚92 and 88 µg⁄1000 individuals respectively ‚compared with 95 µg⁄1000 individuals‚ In untreated control. The effect of M.anisopliae on lipids level in treated extract homogenate appeared decreasing than in untreated one during the period of experiment. The obtained result refer to the mean total lipids were 56‚50‚and 39 µg⁄1000 individuals after24‚48 and 72 hrs ‚respectively compared with59 µg⁄1000 individuals untreated adult females of T. urticae . The results showed biochemical changes in the components of adult females of mite Tetranychus urticae due to the fungus treatment.

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