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El-Badawy, N., Hassan, M., Abd Allah, S., Kamhawy, M. (2012). MORPHOLOGICAL, PHYSIOLOGICAL AND MOLECULAR CHARACTERIZATIONS OF EGYPTIAN Pestalotiopsis ISOLATES. Journal of Plant Protection and Pathology, 3(10), 1105-1119. doi: 10.21608/jppp.2012.84399
Noha F. El-Badawy; Maggie E. M. Hassan; Sahar Sh. Abd Allah; M. A. M. Kamhawy. "MORPHOLOGICAL, PHYSIOLOGICAL AND MOLECULAR CHARACTERIZATIONS OF EGYPTIAN Pestalotiopsis ISOLATES". Journal of Plant Protection and Pathology, 3, 10, 2012, 1105-1119. doi: 10.21608/jppp.2012.84399
El-Badawy, N., Hassan, M., Abd Allah, S., Kamhawy, M. (2012). 'MORPHOLOGICAL, PHYSIOLOGICAL AND MOLECULAR CHARACTERIZATIONS OF EGYPTIAN Pestalotiopsis ISOLATES', Journal of Plant Protection and Pathology, 3(10), pp. 1105-1119. doi: 10.21608/jppp.2012.84399
El-Badawy, N., Hassan, M., Abd Allah, S., Kamhawy, M. MORPHOLOGICAL, PHYSIOLOGICAL AND MOLECULAR CHARACTERIZATIONS OF EGYPTIAN Pestalotiopsis ISOLATES. Journal of Plant Protection and Pathology, 2012; 3(10): 1105-1119. doi: 10.21608/jppp.2012.84399

MORPHOLOGICAL, PHYSIOLOGICAL AND MOLECULAR CHARACTERIZATIONS OF EGYPTIAN Pestalotiopsis ISOLATES

Article 10, Volume 3, Issue 10, October 2012, Page 1105-1119  XML PDF (652.81 K)
Document Type: Original Article
DOI: 10.21608/jppp.2012.84399
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Authors
Noha F. El-Badawy; Maggie E. M. Hassan; Sahar Sh. Abd Allah; M. A. M. Kamhawy
Plant Pathol. Res. Inst., Agric. Res. Cent., Giza, Egypt.
Abstract
Morphological characteristics of authentic cultures of Pestalotiopsis isolates  using scanning electron microscop (SEM) revealed that, no presence of pycnidia for  all examined Pestalotiopsis isolates, that produced acervuli only . Acervuli    size ranged between 350 – 800 μ in length to 270 – 500 μ in width.  Isolates varied in its ability to utilize different carbon sources. There were significant differences in the growth of Pestalotiopsisisolates in different carbon sources containing medium. Maltose gave maximum mean dry weight of mycelial growth. Ammonium nitrate recorded the maximum growth of Pestalotiopsis isolates as the nitrogen source, followed by  potassium nitrate. On the other hand, the minimum mycelia dry weight of the fungus was in Aspartic acid containing media. Sporulation was dense where sodium nitrate was used as the nitrogen source, and deprived when asparagine was used.  The genetic variation between fifteen isolates ofPestalotiopsis spp. were studied using four ITS primers (1&2, 4&5) ) and  SSR 1,2,4,5 and protein electrophoresis.
Keywords
Pestalotiopsis; SEM; carbon sources; nitrogen source; SSR primers; ITS primers; protein electrophoreses
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