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Journal of Plant Protection and Pathology
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El-Kholi, M., Esh, A. (2011). COMPARATIVE STRUCTURAL AND BIOCHEMICAL STUDY ON CALCIUM EFFECTS ON CERCOSPORA LEAF SPOT DISEASE OF SUGAR BEET. Journal of Plant Protection and Pathology, 2(1), 85-97. doi: 10.21608/jppp.2011.84658
M. A. El-Kholi; A. M. H. Esh. "COMPARATIVE STRUCTURAL AND BIOCHEMICAL STUDY ON CALCIUM EFFECTS ON CERCOSPORA LEAF SPOT DISEASE OF SUGAR BEET". Journal of Plant Protection and Pathology, 2, 1, 2011, 85-97. doi: 10.21608/jppp.2011.84658
El-Kholi, M., Esh, A. (2011). 'COMPARATIVE STRUCTURAL AND BIOCHEMICAL STUDY ON CALCIUM EFFECTS ON CERCOSPORA LEAF SPOT DISEASE OF SUGAR BEET', Journal of Plant Protection and Pathology, 2(1), pp. 85-97. doi: 10.21608/jppp.2011.84658
El-Kholi, M., Esh, A. COMPARATIVE STRUCTURAL AND BIOCHEMICAL STUDY ON CALCIUM EFFECTS ON CERCOSPORA LEAF SPOT DISEASE OF SUGAR BEET. Journal of Plant Protection and Pathology, 2011; 2(1): 85-97. doi: 10.21608/jppp.2011.84658

COMPARATIVE STRUCTURAL AND BIOCHEMICAL STUDY ON CALCIUM EFFECTS ON CERCOSPORA LEAF SPOT DISEASE OF SUGAR BEET

Article 9, Volume 2, Issue 1, January 2011, Page 85-97  XML PDF (574.72 K)
Document Type: Original Article
DOI: 10.21608/jppp.2011.84658
Authors
M. A. El-Kholi1; A. M. H. Esh2
1Sugar Cops Research Institute, Agriculture Research Center, Giza, Egypt
2Sugar Crops Research Institute, A.R.C., Giza, Egypt
Abstract
This study was designed to investigate the effect of calcium chloride and calcium chelate on the interaction between sugarbeet plants and Cercospora leaf spot (CLS) disease  as well as its direct effect on the pathogen using scanning electron microscope.  It was clearly observed that, both calcium salts significantly reduced CLS disease severity with calcium chelate being more effective in CLS disease control. The levels of chitinase, β1,3 glucanase and Polyphenol oxidase (PPO) of the salt-treated plants were significantly increased as compared to the untreated control. Thus, both calcium chelate and chloride have a role in triggering the induction of plant resistant. Theincrease ofchitinase, β1,3 glucanase and PPO was correlated with the reduction in disease severity. Scanning electron microscopy showed that, calcium treatments decreased the density of emerging conidiospores compared to those of untreated infected control.The stomatal guard cells of the calcium-treated leaves appeared closure than in the untreated control.  This study concludes that, the two tested calcium salts have a role in inducing disease resistance as well as stomatal functioning. They may also have a fungistatic effect on the pathogen.
Keywords
Cercospora beticola; chitinase; β1; 3 glucanase; Polyphenol oxidase; induce resistance; scanning electron microscope
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