PRELIMINARY EVALUATION OF In vitro SECONDARY METABOLITES OF Sclerotium cepivorum, THE CAUSAL AGENT OF ONION WHITE ROT

El-Sheshtawi, Mohamed; Zaghloul, Mona G.; Elsherbiny, E. A.; Saad, Amany S. M

doi:10.21608/jppp.2013.87473

PRELIMINARY EVALUATION OF In vitro SECONDARY METABOLITES OF Sclerotium cepivorum, THE CAUSAL AGENT OF ONION WHITE ROT

Document Type : Original Article

Authors

¹ Plant Pathology Department, Faculty of Agriculture, Mansoura University, El-Mansoura 35516, Egypt

² Pharmacognosy Department, Faculty of Pharmacy, Mansoura University, El-Mansoura 35516, Egypt

10.21608/jppp.2013.87473

Abstract

Culture filtrates and organic solvent extracts of Sclerotium cepivorum liquid cultures were evaluated for their antifungal activities against the same fungus. Filtrates at 50% taken from 30 to 45-day-old cultures have reduced S. cepivorum growth by 51.9%. This treatment has also caused a reduction in the number of sclerotia by 98.6%. No sclerotial germination was observed on the media amended with the culture filtrates at all concentrations tested (10, 25 and 50%),.Among all organic solvent extracts, methanol extract was the strongest growth inhibitor against the fungal pathogen; it inhibited S. cepivorum growth by 82.2, 93.3, and 93.6% when used at concentrations of 400, 800, and 1200 ppm, respectively. At the same concentrations, ethyl acetate extract exhibited high inhibition to the fungal growth i.e., 85.56, 88.89, and 90.56%, respectively, while chloroform extract was the least effective in this regard causing 71.11% mycelial growth inhibition when used at concentrations of either 800 or 1200 ppm. All organic-solvent extracts completely suppressed the formation and germination of sclerotia at all concentrations. Further studies are needed to isolate different bioactive compounds from secondary metabolites of S. cepivorum.

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