El-Sheshtawi, M., Zaghloul, M., Elsherbiny, E., Saad, A. (2013). PRELIMINARY EVALUATION OF In vitro SECONDARY METABOLITES OF Sclerotium cepivorum, THE CAUSAL AGENT OF ONION WHITE ROT. Journal of Plant Protection and Pathology, 4(8), 721-730. doi: 10.21608/jppp.2013.87473
Mohamed El-Sheshtawi; Mona G. Zaghloul; E. A. Elsherbiny; Amany S. M Saad. "PRELIMINARY EVALUATION OF In vitro SECONDARY METABOLITES OF Sclerotium cepivorum, THE CAUSAL AGENT OF ONION WHITE ROT". Journal of Plant Protection and Pathology, 4, 8, 2013, 721-730. doi: 10.21608/jppp.2013.87473
El-Sheshtawi, M., Zaghloul, M., Elsherbiny, E., Saad, A. (2013). 'PRELIMINARY EVALUATION OF In vitro SECONDARY METABOLITES OF Sclerotium cepivorum, THE CAUSAL AGENT OF ONION WHITE ROT', Journal of Plant Protection and Pathology, 4(8), pp. 721-730. doi: 10.21608/jppp.2013.87473
El-Sheshtawi, M., Zaghloul, M., Elsherbiny, E., Saad, A. PRELIMINARY EVALUATION OF In vitro SECONDARY METABOLITES OF Sclerotium cepivorum, THE CAUSAL AGENT OF ONION WHITE ROT. Journal of Plant Protection and Pathology, 2013; 4(8): 721-730. doi: 10.21608/jppp.2013.87473
PRELIMINARY EVALUATION OF In vitro SECONDARY METABOLITES OF Sclerotium cepivorum, THE CAUSAL AGENT OF ONION WHITE ROT
2Pharmacognosy Department, Faculty of Pharmacy, Mansoura University, El-Mansoura 35516, Egypt
Abstract
Culture filtrates and organic solvent extracts of Sclerotium cepivorum liquid cultures were evaluated for their antifungal activities against the same fungus. Filtrates at 50% taken from 30 to 45-day-old cultures have reduced S. cepivorum growth by 51.9%. This treatment has also caused a reduction in the number of sclerotia by 98.6%. No sclerotial germination was observed on the media amended with the culture filtrates at all concentrations tested (10, 25 and 50%),.Among all organic solvent extracts, methanol extract was the strongest growth inhibitor against the fungal pathogen; it inhibited S. cepivorum growth by 82.2, 93.3, and 93.6% when used at concentrations of 400, 800, and 1200 ppm, respectively. At the same concentrations, ethyl acetate extract exhibited high inhibition to the fungal growth i.e., 85.56, 88.89, and 90.56%, respectively, while chloroform extract was the least effective in this regard causing 71.11% mycelial growth inhibition when used at concentrations of either 800 or 1200 ppm. All organic-solvent extracts completely suppressed the formation and germination of sclerotia at all concentrations. Further studies are needed to isolate different bioactive compounds from secondary metabolites of S. cepivorum.
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