Abd El-latif, A., Mohieldeen, N., Salman, A. (2020). Alpha-Amylase Enzymes of Tribolium castaneum (Herbst) and Callosobruchus maculatus (F.) And Their Response to A-Amylase Inhibitor from Rice أنزبم الألفا أميليز فى حشرتى خنفساء الدقيق الحمراء Tribolium castaneum (Herbst) وخنفساء اللوبيا Callosobruchus maculatus (F.) واستجابتهم لمثبط ألفا أميليز من الأرز. Journal of Plant Protection and Pathology, 11(8), 421-426. doi: 10.21608/jppp.2020.114589
Ashraf Oukasha Abd El-latif; Nessma Mohieldeen; Ahmed Mahmoud Salman. "Alpha-Amylase Enzymes of Tribolium castaneum (Herbst) and Callosobruchus maculatus (F.) And Their Response to A-Amylase Inhibitor from Rice أنزبم الألفا أميليز فى حشرتى خنفساء الدقيق الحمراء Tribolium castaneum (Herbst) وخنفساء اللوبيا Callosobruchus maculatus (F.) واستجابتهم لمثبط ألفا أميليز من الأرز". Journal of Plant Protection and Pathology, 11, 8, 2020, 421-426. doi: 10.21608/jppp.2020.114589
Abd El-latif, A., Mohieldeen, N., Salman, A. (2020). 'Alpha-Amylase Enzymes of Tribolium castaneum (Herbst) and Callosobruchus maculatus (F.) And Their Response to A-Amylase Inhibitor from Rice أنزبم الألفا أميليز فى حشرتى خنفساء الدقيق الحمراء Tribolium castaneum (Herbst) وخنفساء اللوبيا Callosobruchus maculatus (F.) واستجابتهم لمثبط ألفا أميليز من الأرز', Journal of Plant Protection and Pathology, 11(8), pp. 421-426. doi: 10.21608/jppp.2020.114589
Abd El-latif, A., Mohieldeen, N., Salman, A. Alpha-Amylase Enzymes of Tribolium castaneum (Herbst) and Callosobruchus maculatus (F.) And Their Response to A-Amylase Inhibitor from Rice أنزبم الألفا أميليز فى حشرتى خنفساء الدقيق الحمراء Tribolium castaneum (Herbst) وخنفساء اللوبيا Callosobruchus maculatus (F.) واستجابتهم لمثبط ألفا أميليز من الأرز. Journal of Plant Protection and Pathology, 2020; 11(8): 421-426. doi: 10.21608/jppp.2020.114589
Alpha-Amylase Enzymes of Tribolium castaneum (Herbst) and Callosobruchus maculatus (F.) And Their Response to A-Amylase Inhibitor from Rice أنزبم الألفا أميليز فى حشرتى خنفساء الدقيق الحمراء Tribolium castaneum (Herbst) وخنفساء اللوبيا Callosobruchus maculatus (F.) واستجابتهم لمثبط ألفا أميليز من الأرز
Department of Plant protection, Faculty of Agriculture, Sohag University, Egypt
Abstract
Insect α-amylase has recently become a target of insect control strategies using plant-derived α-amylase inhibitors especially for the control of stored product insect pests. The first step to establish such strategy is to characterize the α-amylase enzymes in the target pest. In the current study, α-amylase was biochemically characterized in the larval and adult stages of Tribolium castaneum and Callosobruchus maculatus.T. castaneum was found to have higher α-amylase activity with lower temperature stability compared to that of C. maculatus. The optimum pH for α-amylase activity was 5-6. Zymogram pattern revealed the presence of two α-amylase isoforms with high molecule weight in T. castaneum. C. maculatus has two α-amylase isoforms in the adult stage and three isoforms in the larval stage. Rice α-amylase inhibitors was purified and found to have strong inhibitory effect against α-amylase of both insects and also negatively affected their life parameters.
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