Kandil, M., EL-Shennawy, R., Ahmed, D. (2020). Carbohydrate Hydrolyzing Enzymes As A Target for Pink Bollworm Pectinophora gossypiella (Saund.) Control Agents الانزیمات الهاضمة للکربوهیدرات کهدف لعوامل مکافحة دودة اللوز القرنفلیة Pectinophora gossypiella (Saund.). Journal of Plant Protection and Pathology, 11(10), 493-499. doi: 10.21608/jppp.2020.124902
Mervat A. A. Kandil; Rania M. EL-Shennawy; Dina A. Ahmed. "Carbohydrate Hydrolyzing Enzymes As A Target for Pink Bollworm Pectinophora gossypiella (Saund.) Control Agents الانزیمات الهاضمة للکربوهیدرات کهدف لعوامل مکافحة دودة اللوز القرنفلیة Pectinophora gossypiella (Saund.)". Journal of Plant Protection and Pathology, 11, 10, 2020, 493-499. doi: 10.21608/jppp.2020.124902
Kandil, M., EL-Shennawy, R., Ahmed, D. (2020). 'Carbohydrate Hydrolyzing Enzymes As A Target for Pink Bollworm Pectinophora gossypiella (Saund.) Control Agents الانزیمات الهاضمة للکربوهیدرات کهدف لعوامل مکافحة دودة اللوز القرنفلیة Pectinophora gossypiella (Saund.)', Journal of Plant Protection and Pathology, 11(10), pp. 493-499. doi: 10.21608/jppp.2020.124902
Kandil, M., EL-Shennawy, R., Ahmed, D. Carbohydrate Hydrolyzing Enzymes As A Target for Pink Bollworm Pectinophora gossypiella (Saund.) Control Agents الانزیمات الهاضمة للکربوهیدرات کهدف لعوامل مکافحة دودة اللوز القرنفلیة Pectinophora gossypiella (Saund.). Journal of Plant Protection and Pathology, 2020; 11(10): 493-499. doi: 10.21608/jppp.2020.124902
Carbohydrate Hydrolyzing Enzymes As A Target for Pink Bollworm Pectinophora gossypiella (Saund.) Control Agents الانزیمات الهاضمة للکربوهیدرات کهدف لعوامل مکافحة دودة اللوز القرنفلیة Pectinophora gossypiella (Saund.)
Bollworm Dept., Plant Protection Research Institute, ARC, Dokki, Giza-Egypt.
Abstract
Most insect species depend on the digestive enzymes (invertase, trehalase and amylase) to utilize their uptake of carbohydrates. In the present investigation the activities of these enzymes are used as parameters to study the effect of the selected insecticides [i.e. Teflubenzuron (Nomolt 15%), emamectin benzoate (Proclaim 5% SG); azadirachtin (Saif oil 0.03% EC) and spinosad (Tracer 24% SC)] on pink bollworm, Pectinophora gossypiella. Results showed that invertase had the highest activity (62.03 mg glucose released/gm body weight/min), while amylase had the lowest one (37.91 mg glucose released/gm body weight/min). Treatment caused a reduction effect on the carbohydrate hydrolyzing enzymes, except in the case of trehalase whereas its activity increased significantly only by Saif oil. Tested insecticides could be arranged according to their percentages of changes in the enzymes activities as follows: Nomolt & Tracer > Proclaim > Saif oil. The present study clearly showed that the amylase enzyme appeared to be the most affected enzyme with high level of significant reduction followed by invertase than trehalase, these percentages were -83.42, -48.56 & -17.56; -73.45, +0.70 & -15.55; -19.91, -24.40 & +73.85 and -75.52, -69.01 & 53.55% for Nomolt, Proclaim, Saif oil and Tracer compounds, respectively.
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