Mostafa,, S., EL-Ansary, O., EL-Shemy, A., Ewies, M. (2008). IDENTIFICATION OF THE Paenibaccillus larvae AS A PATHOGEN CAUSATIVE OF (AFB) DISEASE IN HONY BEE COLONIES AND STUDYING THE ANTIBACTERIAL ACTIVITY OF THE PROPOLIS.. Journal of Plant Protection and Pathology, 33(11), 8219-8228. doi: 10.21608/jppp.2008.218968
Shaimaa N. Mostafa,; O. EL-Ansary; A. A. EL-Shemy; M. A. Ewies. "IDENTIFICATION OF THE Paenibaccillus larvae AS A PATHOGEN CAUSATIVE OF (AFB) DISEASE IN HONY BEE COLONIES AND STUDYING THE ANTIBACTERIAL ACTIVITY OF THE PROPOLIS.". Journal of Plant Protection and Pathology, 33, 11, 2008, 8219-8228. doi: 10.21608/jppp.2008.218968
Mostafa,, S., EL-Ansary, O., EL-Shemy, A., Ewies, M. (2008). 'IDENTIFICATION OF THE Paenibaccillus larvae AS A PATHOGEN CAUSATIVE OF (AFB) DISEASE IN HONY BEE COLONIES AND STUDYING THE ANTIBACTERIAL ACTIVITY OF THE PROPOLIS.', Journal of Plant Protection and Pathology, 33(11), pp. 8219-8228. doi: 10.21608/jppp.2008.218968
Mostafa,, S., EL-Ansary, O., EL-Shemy, A., Ewies, M. IDENTIFICATION OF THE Paenibaccillus larvae AS A PATHOGEN CAUSATIVE OF (AFB) DISEASE IN HONY BEE COLONIES AND STUDYING THE ANTIBACTERIAL ACTIVITY OF THE PROPOLIS.. Journal of Plant Protection and Pathology, 2008; 33(11): 8219-8228. doi: 10.21608/jppp.2008.218968
IDENTIFICATION OF THE Paenibaccillus larvae AS A PATHOGEN CAUSATIVE OF (AFB) DISEASE IN HONY BEE COLONIES AND STUDYING THE ANTIBACTERIAL ACTIVITY OF THE PROPOLIS.
1Dept of Economic Entomology. Fac. Of Agric., Alexandria Univ., Egypt
2Dept of Economic Entomology and Pesticides. Fac. Of Agric., Cairo Univ., Giza, Egypt
Abstract
Isolation and identification of the causative pathogen bacteria of the AFB (Paenibaccillus larvae) from the Egyptian honey bee colonies were conducted in this study. The identification was achieved by many tests which assured the pathogen causative. This tests were Roby tests, Holst milk test, selective medium, catalase production, hydrolysis of starch and microscopic examination which involved negative stain, spores staining and Gram stain test. Also a comparison between Egyptian, Bulgarian propolis ethanolic extract and three antibiotics which were tylosin, penicillin and combination of ampicillin and gentamicin from the standpoint of their bacteriostatic effect on three bacterial species which were Paenibaccillus larvae, Listeria monocytogenes and Staphylococcus aureus the comparison was conducted by the technique of measuring the inhibition zone diameter (IZD). The obtained results proved that the Bulgarian propolis ethanolic extract was more effective than local propolis ethanolic extract. Also the S. aureus was more sensitive to propolis ethanolic extract (PEE) than L. monocytogenes, while P. larvae was more resistant for PEE.
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