Document Type : Original Article
Authors
1
Plant Protection Research Intitute, Agricultural Research Center, Al-Sabhia, Alexandria, Egypt
2
Plant Protection Research Institute, Agricultural Research Center, Al-Sabhia, Alexandria, Egypt
3
Agricultural Botany Department, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria 21531, Egypt
Abstract
Counteractions of beta cyclodextrin (βCD) to the light intensity’s impact were investigated on the nutritional indices of Acokanthera spectabilis (Hochst.) extract and chlorfluazuron 5% EC againstthe 4th instar larvae of Spodoptera littoralis (Boisd.). The LC10 value of chlorfluazuron 5% EC (0.92 mg L-1) transcended A. spectabilis extract (0.56 mg L-1) and vice versa for the LC90 values. The most abundant HPLC-compounds of the extract were ferulic acid (2082.49 µg g-1), and ellagic acid (804.74 µg g-1) as well aspalmitic acid (39.89%), and cis-vaccenic acid (14.05%) in GC-MS analysis. There was relative advantage for βCD addition at0.40 gm L-1 to the sub-lethal concentrationsof the tested compounds, observed by their lowest relative potency and highest toxicity index. In darkness, 5000, and 10000 LUX, βCD-mixed extract affected all the larvae’s nutritional indices, likewise βCD-mixed chlorfluazuron 5% EC at 10000 and 20000 LUX excluding dry gained weights, and relative growth rate. Equally highest alkaline phosphatase levels at 48 hrs of exposure to βCD-mixed extract and chlorfluazuron 5% EC were 125.73 and 131.00 U L-1, respectively. Semi-field trials along two seasons showed equally high residual toxicities in net and βCD-mixed extract, as well as highest residual toxicity, and persistency in βCD-mixed chlorfluazuron 5% EC. Spray application of these compounds was safe for the chlorophyll-leaf content, with observed augmentation in all extract treatments. Ultimately, βCD-mixed chlorfluazuron 5% EC could enhance the controlling S. littoralis, while the A. spectabilis extract could be applied alone under light intensity.
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