Toxicity and Nutritional Disruptions Induced by Aspergillus melleus Alkaline Protease in Insect Larvae

Kobisi, A. A.

doi:10.21608/jppp.2024.323481.1267

Home Articles List Article Information

|
|
|
How to cite
RIS EndNote BibTeX APA MLA Harvard Vancouver
|
Share

Journal of Plant Protection and Pathology

Articles in Press

Current Issue

Journal Archive

Volume 16 (2025)

Volume 15 (2024)

Issue 12

Issue 11

Issue 10

Issue 9

Issue 8

Issue 7

Issue 6

Issue 5

Issue 4

Issue 3

Issue 2

Issue 1

Volume 14 (2023)

Volume 13 (2022)

Volume 12 (2021)

Volume 11 (2020)

Volume 10 (2019)

Volume 9 (2018)

Volume 8 (2017)

Volume 7 (2016)

Volume 6 (2015)

Volume 5 (2014)

Volume 4 (2013)

Volume 3 (2012)

Volume 2 (2011)

Volume 1 (2010)

Volume 34 (2009)

Volume 33 (2008)

Volume 32 (2007)

Volume 31 (2006)

Volume 30 (2005)

Volume 29 (2004)

Volume 28 (2003)

Volume 27 (2002)

Volume 26 (2001)

Volume 25 (2000)

	Toxicity and Nutritional Disruptions Induced by Aspergillus melleus Alkaline Protease in Insect Larvae
Article 9, Volume 15, Issue 9, September 2024, Page 299-304 PDF (426.44 K)
Document Type: Original Article
DOI: 10.21608/jppp.2024.323481.1267
View on SCiNiTO
Author
A. A. Kobisi
Plant Protection Department, Desert Research Center, 1 Mathaf El Matariya Street, El Matariya, Cairo, 11753, Egypt
Abstract
This study investigates the insecticidal potential of Aspergillus melleus protease on Galleria mellonella larvae, revealing significant biological effects, including increased mortality, developmental delays, adverse impacts on nutritional indices, and histopathological changes. Fungal spores were isolated from soil in El-Khatatba and cultured to produce protease enzyme, with optimal production occurring at pH 8, 35°C, and 72 hours of incubation. A dose-dependent increase in larval and pupal mortality was observed, with LC₅₀ and LC₉₀ values determined as 1.9 × 10⁶ and 2.5 × 10⁸ spores/mL, respectively. Spore concentrations significantly delayed the larval and pupal development and induced morphological deformities in G. mellonella. Histopathological examination revealed damage to the midgut epithelial layer of the larvae, contributing to impaired nutrient absorption. Nutritional indices revealed a marked decrease in relative growth and consumption rates, as well as reduced conversion efficiency and increased feeding deterrence. These findings highlight the potential of A. melleus protease as a bio-insecticide, with G. mellonella serving as a model insect for evaluating its effectiveness.
Keywords
Entomopathogenic fungi; Protease activity; Insect nutritional indices; Histopathology; Biological Control


Statistics Article View: 112 PDF Download: 122