Evaluation of Cytotoxicity Effects of the Biocontrol Bacterium Bacillus velezensis BE1

Aboelez, Esraa M.; Selim, M. A. E.; Yousef, Safaa A.; Hamza, Safaa; Shabana, Y. M.; Elsherbiny, E. A.

doi:10.21608/jppp.2024.330398.1274

Evaluation of Cytotoxicity Effects of the Biocontrol Bacterium Bacillus velezensis BE1

Document Type : Original Article

Authors

¹ Plant Pathology Department, Faculty of Agriculture, Mansoura University, Mansoura 35516, Egypt

² Agricultural Microbiology Department, Faculty of Agriculture, Mansoura University, Mansoura 35516, Egypt

³ Mycology Research and Plant Disease Survey Department, Plant Pathology Research Institute, Agricultural Research Center, Giza 12619, Egypt

⁴ -Plant Pathology Department, Faculty of Agriculture, Mansoura University, Mansoura 35516, Egypt -National Agricultural and Food Research Council, Academy of Scientific Research and Technology, Cairo, Egypt

10.21608/jppp.2024.330398.1274

Abstract

Bacillus velezensis controls plant pathogens and reduces dependence on synthetic pesticides within sustainable agriculture practices. In the current study, the cell suspension of B. velezensis BE1 did not reduce the viability of Vero cells, kidney cells from an African monkey, HFB4 human skin cell line, or WI-38 cells, which are diploid human fibroblasts generated from the lung cell of a female fetus, at concentrations ranging from 19 × 10¹¹ to 0.59 × 10¹¹. Additionally, the study indicated that cell suspensions of B. velezensis BE1 exhibited no cytotoxicity at any concentration tested on the three cell lines. Cytotoxicity levels ranged between 0 and 1.45 % for Vero cells, 0 and 0.91 % for WI-38 cells, and 0.40 to 6.27 % for HFB4 cells. These findings confirm the biosafety of the endophytic bacterium B. velezensis BE1 and could be used for the control of plant pathogens in both pre- and postharvest diseases.

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