Impact of Antioxidants and Micronutrients as Fungicides Alternatives in Improvment the Antagonism of Trichoderma spp. Against Sclerotinia sclerotiorum

Document Type : Original Article

Authors

1 Mycology Research and Plant Disease Survey Department, Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt.

2 Botany and Microbiology Department, Facuclty of Science, Damanhour University, Egypt.

3 Microbiology Department, Faculty of Science, Zagazig University, Egypt. Running title: Improvement of Trichoderma spp. Antagonism against Sclerotinia sclerotiorum

Abstract

S. sclerotiorum is a strong and resting soil pathogen with high resistance to synthetic fungicides. The biocontrol agent Trichoderma spp.alone provides moderate control of this fungus. In present study, Potassium tartrate, ascorbic acid, folic acid, thiamine and micronutrients mixture were used to improve the number of spores, fresh and dry weights, crude extracts, volatile compounds, and secondary metabolites produced by four isolates of Trichoderma against pathogen. A onetime amendment of thiamine (0.1%) or micronutrients mixture improves the antagonism efficacy of the most Trichoderma isolates showing complete prevention of pathogen growth and inhibition of sclerotia formation.

Keywords