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Journal of Plant Protection and Pathology
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Shams EL Din, A., Azab, M., Abd El-Zaher, T., Mahmoud, H., Sleem, F. (2015). PERSISTENCE OF DINICONAZOLE AND ETOXAZOLE IN BROAD BEAN PODS, PEELS, SEEDS AND ITS PLANTED SOIL. Journal of Plant Protection and Pathology, 6(5), 859-870. doi: 10.21608/jppp.2015.74511
A. M. Shams EL Din; M. M. Azab; Tahany R. Abd El-Zaher; Hend A. Mahmoud; Fatma M. A. Sleem. "PERSISTENCE OF DINICONAZOLE AND ETOXAZOLE IN BROAD BEAN PODS, PEELS, SEEDS AND ITS PLANTED SOIL". Journal of Plant Protection and Pathology, 6, 5, 2015, 859-870. doi: 10.21608/jppp.2015.74511
Shams EL Din, A., Azab, M., Abd El-Zaher, T., Mahmoud, H., Sleem, F. (2015). 'PERSISTENCE OF DINICONAZOLE AND ETOXAZOLE IN BROAD BEAN PODS, PEELS, SEEDS AND ITS PLANTED SOIL', Journal of Plant Protection and Pathology, 6(5), pp. 859-870. doi: 10.21608/jppp.2015.74511
Shams EL Din, A., Azab, M., Abd El-Zaher, T., Mahmoud, H., Sleem, F. PERSISTENCE OF DINICONAZOLE AND ETOXAZOLE IN BROAD BEAN PODS, PEELS, SEEDS AND ITS PLANTED SOIL. Journal of Plant Protection and Pathology, 2015; 6(5): 859-870. doi: 10.21608/jppp.2015.74511

PERSISTENCE OF DINICONAZOLE AND ETOXAZOLE IN BROAD BEAN PODS, PEELS, SEEDS AND ITS PLANTED SOIL

Article 12, Volume 6, Issue 5, May 2015, Page 859-870  XML PDF (529.85 K)
Document Type: Original Article
DOI: 10.21608/jppp.2015.74511
Authors
A. M. Shams EL Din1; M. M. Azab email 1; Tahany R. Abd El-Zaher1; Hend A. Mahmoud2; Fatma M. A. Sleem2
1Plant protection Department, Faculty of Agriculture, Benha University, Egypt
2Central Agricultural Pesticide Laboratory, Agriculture Research Center, Dokki, Giza, Egypt
Abstract
Field experiments were conducted to investigate the persistence of diniconazole and etoxazole in broad bean pods, peels, seeds and soil. Samples were taken after zero, 1, 3, 5, 7, 9, 14 and 21 days after pesticides application. The diniconazole residue in plant samples was extracted by methanol, partition with dichloromethane, cleaned up using coagulation solution, while the soil samples were extracted with ethyl acetate. Estimation of diniconazole residues were performed by (GLC- ECD). Etoxazole was extracted from plant samples with acetone, and from soil samples using acetone: water (8: 2 v/v). The extract was partitioned with dichloromethane and cleaned-up through a florisil column. Determination of etoxazole residues were performed by HPLC-DAD. The recoveries of etoxazole and diniconazole were in the range between 81–99 %. The results indicated that the half–life values of diniconazole in pods, peels, seeds and soil were 2.46, 2.29, 5.25 and 5.56 days, respectively. While these values of etoxazole in pods, peels and soil were 2.27, 1.62 and 4.91 days, respectively. No residues of etoxazole could be detected in seeds. Diniconazole residue was below the MRL in seeds. The pre-harvest interval (PHI) of pods was in diniconazole application 9 days.  And in case of etoxazole these were 11 days.
 
Keywords
persistence; diniconazole; etoxazole; broad bean; PHI; GLC
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