Silver and Magnetic Iron Oxide Nanoparticles-Assisted PCR for the Phytopathogenic Bacteria Ralstonia solanacearum

Shoala, T.; Abd-El-Aziz, Rabab M.

doi:10.21608/jppp.2019.63659

Silver and Magnetic Iron Oxide Nanoparticles-Assisted PCR for the Phytopathogenic Bacteria Ralstonia solanacearum

Document Type : Original Article

Authors

T. Shoala ¹
Rabab M. Abd-El-Aziz ²

¹ Environmental Biotechnology Department, College of Biotechnology, Misr University for Science and Technology, Al-Motamayez District - 6th of October, Egypt

² Plant Bacterial Disease Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt

10.21608/jppp.2019.63659

Abstract

Nanotechnology became an integrated science in different fields. Integration between nanoscience and molecular diagnosis could enhance the early diagnosis of different pathogens. Enhancement of different molecular techniques by using nanomaterials increases the specificity, sensitivity and application of these techniques in biology. Polymerase chain reaction (PCR) is considered as one of the main basic molecular techniques that could be enhanced by using nanomaterials. Silver (Ag) and magnetic iron oxide (Fe₂O₃) nanoparticles were chemically synthesized and characterized by using transmission electron microscope (TEM) according to standard protocol. The sizes of Ag and magnetic Fe₂O₃ nanoparticles were ∼15-20 and ~12-23 nm diameters respectively. DNA template of Ralstonia solanacearum was extracted by using Ag and magnetic Fe₂O₃ nanoparticles separately and in mixture of both nanomaterials in addition to the control. Extraction of DNA template by using combined nanomaterials increased the DNA yield followed by significant augmentation of the PCR efficacy for small concentration of bacterial DNA template. Inspections of the mechanism of such PCR augmentation suggested an indication of quick transfer of heat in the presence of Ag and magnetic Fe₂O₃ nanoparticles. Silver and magnetic Fe₂O₃ nanoparticles-supported PCR could be applicable for reducing the total PCR cycles and enhancing the augmentation of DNA amplicons from a diversity of samples, as well as GC-rich patterns (such as the bacterial genome of R. solanacearum) that are frequently observed to yield disappointing results.

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